JBO Letters

Complementary optical and nuclear imaging of caspase-3 activity using combined activatable and radio-labeled multimodality molecular probe

[+] Author Affiliations
Hyeran Lee, Walter J. Akers, Philip P. Cheney, W. Barry Edwards, Kexian Liang, Joseph P. Culver

Washington University, School of Medicine, Department of Radiology, St. Louis, Missouri 63110

Samuel Achilefu

Washington University, School of Medicine, Department of Radiology, St. Louis, Missouri 63110

Washington University, Department of Biochemistry and Molecular Biophysics, St. Louis, Missouri 63110

J. Biomed. Opt. 14(4), 040507 (August 25, 2009). doi:10.1117/1.3207156
History: Received May 22, 2009; Revised June 26, 2009; Accepted June 30, 2009; Published August 25, 2009
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* Address all correspondence to: Samuel Achilefu, E-mail: achilefus@mir.wustl.edu

Based on the capability of modulating fluorescence intensity by specific molecular events, we report a new multimodal optical-nuclear molecular probe with complementary reporting strategies. The molecular probe (LS498) consists of tetraazacyclododecanetetraacetic acid (DOTA) for chelating a radionuclide, a near-infrared fluorescent dye, and an efficient quencher dye. The two dyes are separated by a cleavable peptide substrate for caspase-3, a diagnostic enzyme that is upregulated in dying cells. LS498 is radiolabeled with Cu64, a radionuclide used in positron emission tomography. In the native form, LS498 fluorescence is quenched until caspase-3 cleavage of the peptide substrate. Enzyme kinetics assay shows that LS498 is readily cleaved by caspase-3, with excellent enzyme kinetic parameters kcat and KM of 0.55±0.01s1 and 1.12±0.06μM, respectively. In mice, the initial fluorescence of LS498 is ten-fold less than control. Using radiolabeled Cu64-LS498 in a controlled and localized in-vivo model of caspase-3 activation, a time-dependent five-fold NIR fluorescence enhancement is observed, but radioactivity remains identical in caspase-3 positive and negative controls. These results demonstrate the feasibility of using radionuclide imaging for localizing and quantifying the distribution of molecular probes and optical imaging for reporting the functional status of diagnostic enzymes.

Figures in this Article

Multimodal imaging has been driven by the realization that no single imaging method has a complete solution to the multifaceted challenges of disease diagnosis and prognosis. For example, the addition of molecular imaging to the current functional and structural imaging methods requires procedures that report molecular events without loss of anatomical information. A common practice today is to combine methods with high spatial resolution such as magnetic resonance imaging (MRI), x-ray computed tomography (CT), and ultrasound with those with high detection sensitivity such as positron emission tomography (PET) and diffuse optical tomography (DOT).

A less utilized multimodal strategy is the combination of two functional or molecular imaging methods, such as optical-nuclear multimodal imaging. As summarized in a recent report, there are many reasons to utilize this hybrid of two modalities with similarly high detection sensistivity.1 For example, although combining molecular optical contrasts with MRI or CT provides coregistered reference anatomy, the disparate contrast mechanisms present a barrier to integrating the imaging data. Because of the high sensitivity of both PET and optical methods and the compatibility of their imaging agents, we and others have reported the use of monomolecular multimodality imaging agents (MOMIAs) for combined nuclear-optical imaging studies.23 These studies have established the equivalence or identical origin of signals from both contrast sources through image coregistration. The dual optical-nuclear imaging approach has been extended to nanomaterials, where PET and optical imaging have been combined to improve quantitative accuracy.45

In this study, we sought to develop a complementary imaging strategy that would harness the strengths of NIR fluorescence and nuclear imaging methods. To achieve this goal, we hypothesized that incorporating an activatable fluorescent molecular system into a radio-labeled cleavable peptide would provide a unique opportunity to fuse imaging data with identical pharmacokinetics but different reporting strategies. Accordingly, we prepared a multifunctional molecular probe (LS498) with persistent radionuclide signal and activatable fluorescence in response to a specific molecular process.

The peptide backbone, metal chelating group DOTA, and NIR fluorescent dye6 used in LS498 (Fig. 1), were assembled as described previously.2 The NIR fluorescent quencher, IR dye QC-1 (Licor, Lincoln, Nebraska), was incorporated into the multifunctional peptide at room temperature in phosphate buffered saline (PBS) for 24h. The purified LS498 (5μg, 1.3nmol) was radio-labeled by heating (60°C, 0.5h) with C64u(470μCi) in aqueous buffer (100-mM NH4OAc, pH 5.5). The caspase-3 enzyme kinetic parameters were determined as previously described.78

Graphic Jump LocationF1 :

Structure of caspase-3 activatable optical-nuclear molecular probe (LS498).

As a model of tumor-related caspase activity, plastic tubes containing C64u-LS498 (50μL, 1μM) and either 260-pM caspase-3 or 5-μM bovine serum albumin (BSA) in assay buffer were implanted subcutaneously in opposite flanks of the mouse. Multimodal imaging and region of interest (ROI) analysis of fluorescence (755-nm excitation, 830-nm emission), x-ray, and scintigraphy were performed with the IS4000MM (Carestream Health, New Haven, Connecticut) as previously described.2

Development of Caspase-3 Activatable Probe for Dual Optical-Nuclear Imaging

A multifunctional peptide-based molecular probe LS498 (Fig. 1) was designed and prepared for use in this study. Because of the need to monitor the response of diseased tissue to treatment and the implication of caspase-3 in early cell death,7 LS498 was specifically developed to report the activity of this diagnostic enzyme. To accomplish this goal, we used a fluorescence resonance energy transfer (FRET) system, where the fluorescence of a NIR dye was efficiently quenched with wide-spectrum quencher dye. Since the tetrapeptide sequence, aspartic acid-glutamic-acid-valine-aspartic acid (DEVD), is an established substrate for caspases-3,7 we incorporated this peptide sequence between the two dyes. Cleavage of the DEVD peptide results in fluorescence enhancement that can be used to monitor enzyme activity.78 In the quenched state, it is not feasible to image the distribution of the molecular probe in tissue prior to enzyme cleavage. Moreover, lack of fluorescence enhancement may be due to inadequate delivery of the molecular probe to the target tissue, a situation that could result in false-negative outcomes. To address this issue, LS498 was labeled with C64u, a positron emitter with half-life of 12.8h. This radionuclide is widely used in PET imaging of molecular processes in small animals and humans.9 After HPLC purification, LS498 was labeled with Cu64 at high specific activity (360Cimmol) and purity (>99%). In previous work with Cu64-DOTA-c(RGDyK), specific activities ranged from 200to500Cimmol and receptor-specific tumor accumulation allowed for αvβ3–positive tumor visualization by small animal PET.10 Therefore, the observed specific activity in LS498 is adequate for receptor targeted tumor imaging in vivo.

Caspase-3 Enzyme Kinetics

The feasibility of applying a reporter of proteolytic activity to in-vivo imaging depends on how fast the substrate is processed by the enzyme before being washed away from the target site. The kinetic parameters, kcat and KM, are measurable indicators of how well a substrate is processed by an enzyme. Our study shows that LS498 was readily cleaved by caspase-3 and displayed classic Michaelis-Menten kinetics (Fig. 2) with enzyme kinetic parameters kcat and KM of 0.55±0.01s1 and 1.12±0.06μM, respectively. The observed kcat and KM compares favorably with standard substrates Ac-DEVD-AMC (kcat=0.75s1, KM=9.7μM) and Ac-DEVD-pNA (kcat=0.55±0.01s1, KM=11μM). The kcatKM ratio, which measures the performance constant of an enzyme for a substrate, was found to be 4.91×105s1M1.

Graphic Jump LocationF2 :

Nonlinear fit of initial velocity with respect to substrate concentration and the corresponding Lineweaver-Burk plot (insert). Substrate concentrations varied from 270nMto35μM.

In-Vivo Imaging of LS-498 Distribution and Model of Caspase-3 Activation

LS498 was designed to prevent fluorescence emission prior to activation by caspase-3. To assess if this goal was met, we compared the fluorescence emission of LS498 to a control analog. The control peptide lacks a quencher dye, thereby reporting the maximum fluorescence intensity of a completely cleaved LS498. Intravenous injection of the two molecular probes in healthy mice showed that fluorescence was hardly detectable in the mouse injected with LS498 and remained lower than that of the control probe up to 24-h postinjection. Immediately after injection (30min), the fluorescence intensity was at least ten-fold less for LS498 relative to control [Fig. 3]. After 24h, both molecular probes had similar low fluorescence intensity (data not shown), approaching the detection limit of our imaging system. Interestingly, the kidneys were visible at 24h in both mice, suggesting a possible degradation of LS498 after prolonged retention in this organ. Alternatively, it is also possible that the kidneys express residual levels of caspase-3 that were responsible for the observed fluorescence enhancement.

Graphic Jump LocationF3 :

Imaging of multifunctional molecular probe in mice. (a) In-vivo distribution of LS498 (right) and nonquenched control analog (left) in mice at 30min after intravenous injection of the imaging agents. Fluorescence of the activatable probe was efficiently quenched by greater than 10-fold relative to the nonquenched control. (b) Multimodal fluorescence (left) and scintigraphic (right) imaging of a mouse with subcutaneously implanted tubes containing Cu64-LS498 with BSA (left side) or caspase-3 (right side) 2h after implantation. Tubes were implanted subcutaneously (about 1mm) below the surface of the skin. Radioactivity is always “on” but fluorescence enhancement depends on the presence of caspase-3. The ratio of fluorescence intensity for the caspase-3 sample was 4.2 times greater than the control sample, and this ratio increased to 5.6-fold after normalizing to radioactivity.

To evaluate the feasibility of imaging caspase-3 activity in small animals, we developed an artificial model of subcutaneous tumor in mice. Two tubes containing radiolabeled Cu64-LS498 were each mixed with either caspase-3 or bovine serum albumin (BSA) to mimic tissues expressing caspase-3 versus control, respectively. Expectedly, the fluorescence of caspase-3 containing tube increased with time, reaching a plateau after 4h. In contrast, the BSA control tube did not show any fluorescence enhancement up to 24h [Fig. 3]. In contrast to the scintigraphy image where both tubes had similar radioactivity, the optical imaging clearly distinguished caspase-3 positive from the negative tube based on differences in their fluorescence intensity. These findings demonstrate the feasibility of using LS498 to report concentration through nuclear contrast and enzyme activity through optical contrast in a controlled in-vivo context. To be effective in a tumor model, LS498 has to be internalized in cells where the cytosolic caspase-3 resides. Cytosolic delivery could be achieved by incorporating cell-permeating peptides to LS498 or via a receptor-mediated endocytosis mechanism that is coupled with endosomal disrupting peptides.

In conclusion, this work summarizes our ongoing efforts toward the development of multimodality imaging agents for combined optical and nuclear molecular imaging of diseased tissues. In this study, the always “on” nuclear signal is useful for quantifying and localizing the distribution of the probe, while the optical imaging reports the functional status of a target molecular event. Both in-vitro and in-vivo results demonstrate the feasibility of using this approach to image molecular processes. Although we used caspase-3 as a model for this study, the complementary contrast strategy is applicable to imaging the functional status of most enzymes. Studies are in progress to demonstrate the utility of this new imaging strategy in animal disease models.

We thank Licor for the generous gift of the quencher dye used in this study. This study was funded in part by the National Institutes of Health (R01 CA109754 and R01EB008458).

Culver  J., , Akers  W., , and Achilefu  S., “ Multimodality molecular imaging with combined optical and SPECT/PET modalities. ,” J. Nucl. Med..  0161-5505 49, , 169–172  ((2008)).
Edwards  W. B., , Akers  W. J., , Ye  Y., , Cheney  P. P., , Bloch  S., , Xu  B., , Laforest  R., , and Achilefu  S., “ Multimodal imaging of integrin receptor-positive tumors by bioluminescence, fluorescence, gamma scintigraphy, and single-photon emission computed tomography using a cyclic RGD peptide labeled with a near-infrared fluorescent dye and a radionuclide. ,” Mol. Imaging.  1535-3508 8, , 101–110  ((2009)).
Li  C., , Wang  W., , Wu  Q., , Ke  S., , Houston  J., , Sevick-Muraca  E., , Dong  L., , Chow  D., , Charnsangavej  C., , and Gelovani  J. G., “ Dual optical and nuclear imaging in human melanoma xenografts using a single targeted imaging probe. ,” Nucl. Med. Biol..  0969-8051 33, , 349–358  ((2006)).
Cai  W., , Chen  K., , Li  Z. B., , Gambhir  S. S., , and Chen  X., “ Dual-function probe for PET and near-infrared fluorescence imaging of tumor vasculature. ,” J. Nucl. Med..  0161-5505 48, , 1862–1870  ((2007)).
Duconge  F., , Pons  T., , Pestourie  C., , Herin  L., , Theze  B., , Gombert  K., , Mahler  B., , Hinnen  F., , Kuhnast  B., , Dolle  F., , Dubertret  B., , and Tavitian  B., “ Fluorine-18-labeled phospholipid quantum dot micelles for in vivo multimodal imaging from whole body to cellular scales. ,” Bioconjugate Chem..  1043-1802 19, , 1921–1926  ((2008)).
Lee  H., , Berezin  M. Y., , Henary  M., , Strekowski  L., , and Achilefu  S., “ Fluorescence lifetime properties of near-infrared cyanine dyes in relation to their structures. ,” J. Photochem. Photobiol., A.  1010-6030 200, , 438–444  ((2008)).
Bullok  K. E., , Maxwell  D., , Kesarwala  A. H., , Gammon  S., , Prior  J. L., , Snow  M., , Stanley  S., , and Piwnica-Worms  D., “ Biochemical and in vivo characterization of a small, membrane-permeant, caspase-activatable far-red fluorescent peptide for imaging apoptosis. ,” Biochem. J..  0264-6021 46, , 4055–4065  ((2007)).
Zhang  Z., , Fan  J., , Cheney  P. P., , Berezin  M. Y., , Edwards  W. B., , Akers  W. J., , Shen  D., , Liang  K., , Culver  J. P., , and Achilefu  S., “ Activatable molecular systems using homologous near-infrared fluorescent probes for monitoring enzyme activities in vitro, in cellulo, and in vivo. ,” Mol. Pharmaceutics. 6, , 416–427  ((2009)).
Lewis  M. R., , Wang  M., , Axworthy  D. B., , Theodore  L. J., , Mallet  R. W., , Fritzberg  A. R., , Welch  M. J., , and Anderson  C. J., “ In vivo evaluation of pretargeted 64Cu for tumor imaging and therapy. ,” J. Nucl. Med..  0161-5505 44, , 1284–1292  ((2003)).
Chen  X., , Park  R., , Tohme  M., , Shahinian  A. H., , Bading  J. R., , and Conti  P. S., “ MicroPET and autoradiographic imaging of breast cancer alpha v-integrin expression using 18F- and 64Cu-labeled RGD peptide. ,” Bioconjugate Chem..  1043-1802 15, , 41–49  ((2004)).
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Hyeran Lee ; Walter J. Akers ; Philip P. Cheney ; W. Barry Edwards ; Kexian Liang, et al.
"Complementary optical and nuclear imaging of caspase-3 activity using combined activatable and radio-labeled multimodality molecular probe", J. Biomed. Opt. 14(4), 040507 (August 25, 2009). ; http://dx.doi.org/10.1117/1.3207156


Figures

Graphic Jump LocationF1 :

Structure of caspase-3 activatable optical-nuclear molecular probe (LS498).

Graphic Jump LocationF2 :

Nonlinear fit of initial velocity with respect to substrate concentration and the corresponding Lineweaver-Burk plot (insert). Substrate concentrations varied from 270nMto35μM.

Graphic Jump LocationF3 :

Imaging of multifunctional molecular probe in mice. (a) In-vivo distribution of LS498 (right) and nonquenched control analog (left) in mice at 30min after intravenous injection of the imaging agents. Fluorescence of the activatable probe was efficiently quenched by greater than 10-fold relative to the nonquenched control. (b) Multimodal fluorescence (left) and scintigraphic (right) imaging of a mouse with subcutaneously implanted tubes containing Cu64-LS498 with BSA (left side) or caspase-3 (right side) 2h after implantation. Tubes were implanted subcutaneously (about 1mm) below the surface of the skin. Radioactivity is always “on” but fluorescence enhancement depends on the presence of caspase-3. The ratio of fluorescence intensity for the caspase-3 sample was 4.2 times greater than the control sample, and this ratio increased to 5.6-fold after normalizing to radioactivity.

Tables

References

Culver  J., , Akers  W., , and Achilefu  S., “ Multimodality molecular imaging with combined optical and SPECT/PET modalities. ,” J. Nucl. Med..  0161-5505 49, , 169–172  ((2008)).
Edwards  W. B., , Akers  W. J., , Ye  Y., , Cheney  P. P., , Bloch  S., , Xu  B., , Laforest  R., , and Achilefu  S., “ Multimodal imaging of integrin receptor-positive tumors by bioluminescence, fluorescence, gamma scintigraphy, and single-photon emission computed tomography using a cyclic RGD peptide labeled with a near-infrared fluorescent dye and a radionuclide. ,” Mol. Imaging.  1535-3508 8, , 101–110  ((2009)).
Li  C., , Wang  W., , Wu  Q., , Ke  S., , Houston  J., , Sevick-Muraca  E., , Dong  L., , Chow  D., , Charnsangavej  C., , and Gelovani  J. G., “ Dual optical and nuclear imaging in human melanoma xenografts using a single targeted imaging probe. ,” Nucl. Med. Biol..  0969-8051 33, , 349–358  ((2006)).
Cai  W., , Chen  K., , Li  Z. B., , Gambhir  S. S., , and Chen  X., “ Dual-function probe for PET and near-infrared fluorescence imaging of tumor vasculature. ,” J. Nucl. Med..  0161-5505 48, , 1862–1870  ((2007)).
Duconge  F., , Pons  T., , Pestourie  C., , Herin  L., , Theze  B., , Gombert  K., , Mahler  B., , Hinnen  F., , Kuhnast  B., , Dolle  F., , Dubertret  B., , and Tavitian  B., “ Fluorine-18-labeled phospholipid quantum dot micelles for in vivo multimodal imaging from whole body to cellular scales. ,” Bioconjugate Chem..  1043-1802 19, , 1921–1926  ((2008)).
Lee  H., , Berezin  M. Y., , Henary  M., , Strekowski  L., , and Achilefu  S., “ Fluorescence lifetime properties of near-infrared cyanine dyes in relation to their structures. ,” J. Photochem. Photobiol., A.  1010-6030 200, , 438–444  ((2008)).
Bullok  K. E., , Maxwell  D., , Kesarwala  A. H., , Gammon  S., , Prior  J. L., , Snow  M., , Stanley  S., , and Piwnica-Worms  D., “ Biochemical and in vivo characterization of a small, membrane-permeant, caspase-activatable far-red fluorescent peptide for imaging apoptosis. ,” Biochem. J..  0264-6021 46, , 4055–4065  ((2007)).
Zhang  Z., , Fan  J., , Cheney  P. P., , Berezin  M. Y., , Edwards  W. B., , Akers  W. J., , Shen  D., , Liang  K., , Culver  J. P., , and Achilefu  S., “ Activatable molecular systems using homologous near-infrared fluorescent probes for monitoring enzyme activities in vitro, in cellulo, and in vivo. ,” Mol. Pharmaceutics. 6, , 416–427  ((2009)).
Lewis  M. R., , Wang  M., , Axworthy  D. B., , Theodore  L. J., , Mallet  R. W., , Fritzberg  A. R., , Welch  M. J., , and Anderson  C. J., “ In vivo evaluation of pretargeted 64Cu for tumor imaging and therapy. ,” J. Nucl. Med..  0161-5505 44, , 1284–1292  ((2003)).
Chen  X., , Park  R., , Tohme  M., , Shahinian  A. H., , Bading  J. R., , and Conti  P. S., “ MicroPET and autoradiographic imaging of breast cancer alpha v-integrin expression using 18F- and 64Cu-labeled RGD peptide. ,” Bioconjugate Chem..  1043-1802 15, , 41–49  ((2004)).

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