Research Papers

Infrared-based third and second harmonic generation imaging of cornea

[+] Author Affiliations
Szu-Yu Chen

National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Department of Electrical Engineering, No 1 Roosevelt Road Section 4, Taipei 10617, Taiwan

Han-Chieh Yu, I-Jong Wang

National Taiwan University Hospital, Graduate Institute of Pathology, Department of Pathology, Taipei 100, Taiwan

Chi-Kuang Sun

National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Department of Electrical Engineering, No 1 Roosevelt Road Section 4, Taipei 10617, Taiwan and Academia Sinica, Research Center for Applied Sciences, Taipei 115, Taiwan

J. Biomed. Opt. 14(4), 044012 (August 25, 2009). doi:10.1117/1.3183805
History: Received November 03, 2008; Revised May 24, 2009; Accepted May 27, 2009; Published August 25, 2009
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The cornea functions as an optical lens and plays an important role in vision. For corneal diagnosis and treatment such as refractive surgery, a microscopic imaging system with a 3-D cellular resolution and retinal safety is strongly desired. Recently, confocal and multiphoton microscopies have been applied to corneal imaging with visible to near-infrared light sources. To increase retinal safety, an infrared light source is be needed. In this work, an infrared-based third and second harmonic generation microscopic study of mouse eyes is reported with 700-μm penetrability and high cellular resolution. This study provides a critical reference for future development of infrared-based corneal imaging.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Szu-Yu Chen ; Han-Chieh Yu ; I-Jong Wang and Chi-Kuang Sun
"Infrared-based third and second harmonic generation imaging of cornea", J. Biomed. Opt. 14(4), 044012 (August 25, 2009). ; http://dx.doi.org/10.1117/1.3183805


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