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Research Papers

Theoretical simulation study of linearly polarized light on microscopic second-harmonic generation in collagen type I

[+] Author Affiliations
Ying Chang, Changshui Chen

South China Normal University, MOE Key Laboratory of Laser Life Science, Shipai, Guangzhou, Guangdong 510631, China

Jianxin Chen

Fujian Normal University, Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, No. 8 Shangshan Road, Fuzhou, Fujian 350007, China

Ying Jin, Xiaoyuan Deng

South China Normal University, MOE Key Laboratory of Laser Life Science, Shipai, Guangzhou, 510631, China

J. Biomed. Opt. 14(4), 044016 (July 27, 2009). doi:10.1117/1.3174427
History: Received January 15, 2009; Revised May 11, 2009; Accepted May 28, 2009; Published July 27, 2009
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A theoretical model is established for dealing with second-harmonic generation (SHG) in type I collagen excited by linearly polarized light focused by a microscope. With this model, the effects of the polarization angle α, numerical aperture (NA), as well as the ratio of hyperpolarizability ρ=βxxxβxyy on SHG emission have been investigated. Simulation results reveal that SHG emission power changes periodically as α. The use of lower NA leads to weaker SHG emission but is more concentrated in two closer lobes, whereas more distributed emission in two detached lobes appear at higher NA. As the introduction of polarization direction, which is not along with the fiber axis (α0deg), one more element βxyy is valid in our case than βxxx alone, while their ratio ρ plays a very important role for collagen features characterization. SHG emission with ρ shows complicated modality that SHG emission is different at different α and not symmetric at ±ρ except at α=0deg, suggesting the important impact of polarization working on ρ for SHG emission. Our theoretical simulation results provide useful clues for experimental study of microscopic SHG emission in collagen excited by linearly polarized beam.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Ying Chang ; Changshui Chen ; Jianxin Chen ; Ying Jin and Xiaoyuan Deng
"Theoretical simulation study of linearly polarized light on microscopic second-harmonic generation in collagen type I", J. Biomed. Opt. 14(4), 044016 (July 27, 2009). ; http://dx.doi.org/10.1117/1.3174427


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