Research Papers

Effect of anatomy on spectroscopic detection of cervical dysplasia

[+] Author Affiliations
Jelena Mirkovic, Condon Lau, Sasha McGee, Chung-Chieh Yu, Jonathan Nazemi, Luis Galindo

Massachusetts Institute of Technology, George R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139

Victoria Feng

Boston Medical Center, Department of Obstetrics and Gynecology, 85 East Concord Street, Boston, Massachusetts 02118

Teresa Darragh

University of California, San Francisco, Department of Pathology, 1600 Divisadero, Room B618, San Francisco, California 94115

Antonio de las Morenas

Boston Medical Center, Department of Pathology, 670 Albany Street, Biosquare III Boulevard, Boston, Massachusetts 02118

Christopher Crum

Brigham and Women’s Hospital, Department of Pathology, 75 Francis Street, Boston, Massachusetts 02115

Elizabeth Stier

Boston Medical Center, Department of Obstetrics and Gynecology, 85 East Concord Street, Boston, Massachusetts 02118

Michael Feld, Kamran Badizadegan

Massachusetts Institute of Technology, George R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139

J. Biomed. Opt. 14(4), 044021 (September 01, 2009). doi:10.1117/1.3194142
History: Received February 28, 2009; Revised June 03, 2009; Accepted June 04, 2009; Published September 01, 2009
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It has long been speculated that underlying variations in tissue anatomy affect in vivo spectroscopic measurements. We investigate the effects of cervical anatomy on reflectance and fluorescence spectroscopy to guide the development of a diagnostic algorithm for identifying high-grade squamous intraepithelial lesions (HSILs) free of the confounding effects of anatomy. We use spectroscopy in both contact probe and imaging modes to study patients undergoing either colposcopy or treatment for HSIL. Physical models of light propagation in tissue are used to extract parameters related to tissue morphology and biochemistry. Our results show that the transformation zone, the area in which the vast majority of HSILs are found, is spectroscopically distinct from the adjacent squamous mucosa, and that these anatomical differences can directly influence spectroscopic diagnostic parameters. Specifically, we demonstrate that performance of diagnostic algorithms for identifying HSILs is artificially enhanced when clinically normal squamous sites are included in the statistical analysis of the spectroscopic data. We conclude that underlying differences in tissue anatomy can have a confounding effect on diagnostic spectroscopic parameters and that the common practice of including clinically normal squamous sites in cervical spectroscopy results in artificially improved performance in distinguishing HSILs from clinically suspicious non-HSILs.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Jelena Mirkovic ; Condon Lau ; Sasha McGee ; Chung-Chieh Yu ; Jonathan Nazemi, et al.
"Effect of anatomy on spectroscopic detection of cervical dysplasia", J. Biomed. Opt. 14(4), 044021 (September 01, 2009). ; http://dx.doi.org/10.1117/1.3194142


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