Research Papers

Monte Carlo characterization of parallelized fluorescence confocal systems imaging in turbid media

[+] Author Affiliations
Anthony A. Tanbakuchi

University of Arizona, Department of Radiology, Radiology Research Laboratories, 1609 North Warren Avenue, Tucson, Arizona 85724 and University of Arizona, College of Optical Sciences, 1630 East University Boulevard, Tucson, Arizona 85721

Andrew R. Rouse

University of Arizona, Department of Radiology, Radiology Research Laboratories, 1609 North Warren Avenue, Tucson, Arizona 85724

Arthur F. Gmitro

University of Arizona, Department of Radiology, Radiology Research Laboratories, 1609 North Warren Avenue, Tucson, Arizona 85724 and University of Arizona, College of Optical Sciences, 1630 East University Boulevard, Tucson, Arizona 85721

J. Biomed. Opt. 14(4), 044024 (August 20, 2009). doi:10.1117/1.3194131
History: Received April 13, 2009; Revised May 08, 2009; Accepted May 08, 2009; Published August 20, 2009
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We characterize and compare the axial and lateral performance of fluorescence confocal systems imaging in turbid media. The aperture configurations studied are a single pinhole, a slit, a Nipkow disk, and a linear array of pinholes. Systems with parallelized apertures are used clinically because they enable high-speed and real-time imaging. Understanding how they perform in highly scattering tissue is important. A Monte Carlo model was developed to characterize parallelized system performance in a scattering media representative of human tissues. The results indicate that a slit aperture has degraded performance, both laterally and axially. In contrast, the analysis reveals that multipinhole apertures such as a Nipkow disk or a linear pinhole array can achieve performance nearly equivalent to a single pinhole aperture. The optimal aperture spacing for the multipinhole apertures was determined for a specific tissue model. In addition to comparing aperture configurations, the effects of tissue nonradiative absorption, scattering anisotropy, and fluorophore concentration on lateral and axial performance of confocal systems were studied.

© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Anthony A. Tanbakuchi ; Andrew R. Rouse and Arthur F. Gmitro
"Monte Carlo characterization of parallelized fluorescence confocal systems imaging in turbid media", J. Biomed. Opt. 14(4), 044024 (August 20, 2009). ; http://dx.doi.org/10.1117/1.3194131


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