Research Papers

Improved diagnosis of oral premalignant lesions in submucous fibrosis patients with 5-aminolevulinic acid induced PpIX fluorescence

[+] Author Affiliations
Chih-Yu Wang

I-Shou University, Department of Biomedical Engineering, No. 1 Hsueh-Cheng Road, Section 1, Tahsu Hsiang, Kaushiung 840, Taiwan

Tsuimin Tsai

Taipei Medical University, Graduate Institute of Biomedical Materials and Engineering, Taipei, 110, Taiwan

Chung-Ping Chiang, Hsin-Ming Chen

National Taiwan University, School of Dentistry, Taipei, 100, Taiwan

Chin-Tin Chen

National Taiwan University College of Medicine, Center for Optoelectronic Biomedicine, No. 1 Jen-Ai Road, Section 1, Taipei, 10051, Taiwan and National Taiwan University, Department of Biochemical Science and Technology, Taipei, 106, Taiwan

J. Biomed. Opt. 14(4), 044026 (August 07, 2009). doi:10.1117/1.3200934
History: Received December 29, 2008; Revised April 26, 2009; Accepted June 16, 2009; Published August 07, 2009
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We investigate the possibility of using ALA-derived PpIX fluorescence spectroscopy for the detection of epithelial hyperkeratosis (EH) or epithelial dysplasia (ED) lesions in oral submucous fibrosis (OSF) patients that could not be found by autofluorescence spectroscopy. Twenty percent of ALA solution gel was applied onto oral neoplasia and surrounding normal tissue [normal oral mucosa (NOM)] for 90min. Fluorescence emission spectra were measured under 410nm excitation. Generally, the most intense fluorescence emission peaks occurred at 460 and 630nm. The ratios of the area under red peak (630±10nm) to the area under blue peak (460±10nm), denoted as RB, were calculated. We found that OSF mucosa has the lowest RB value, followed by NOM, EH on OSF, and ED on OSF. An ANOVA test showed significant differences between OSF, NOM, EH on OSF, and ED on OSF (p<0.05). However, measurements of autofluorescence (i.e., before ALA application) show no significant differences between OSF, NOM, EH on OSF, and ED on OSF (ANOVA test, p>0.05). These results indicate that ALA-induced PpIX fluorescence spectroscopy could be used to identify the premalignant lesions on oral fibrotic mucosa, which could not be found by autofluorescence.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Chih-Yu Wang ; Tsuimin Tsai ; Chung-Ping Chiang ; Hsin-Ming Chen and Chin-Tin Chen
"Improved diagnosis of oral premalignant lesions in submucous fibrosis patients with 5-aminolevulinic acid induced PpIX fluorescence", J. Biomed. Opt. 14(4), 044026 (August 07, 2009). ; http://dx.doi.org/10.1117/1.3200934


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