Research Papers

Effects of a topically applied wound ointment on epidermal wound healing studied by in vivo fluorescence laser scanning microscopy analysis

[+] Author Affiliations
Bernhard Lange-Asschenfeldt

Charité-Universitätsmedizin Berlin, University Medical School, Department of Dermatology, Venerology and Allergology and Skin Cancer Center, Berlin, 10117, Germany

Alena Alborova

Charité-Universitätsmedizin Berlin, University Medical School, Department of Dermatology, Venerology and Allergology and Center of Experimental and Applied Skin Physiology, Berlin, 10117, Germany

Daniela Krüger-Corcoran

Charité-Universitätsmedizin Berlin, University Medical School, Department of Dermatology, Venerology and Allergology and Skin Cancer Center, Berlin, 10117, Germany

Alexa Patzelt, Heike Richter

Charité-Universitätsmedizin Berlin, University Medical School, Department of Dermatology, Venerology and Allergology and Center of Experimental and Applied Skin Physiology, Berlin, 10117, Germany

Wolfram Sterry

Charité-Universitätsmedizin Berlin, University Medical School, Department of Dermatology, Venerology and Allergology, Berlin, 10117, Germany

Axel Kramer

Ernst Moritz Arndt University Greifswald, Institute of Hygiene and Environmental Medicine, Greifswald, 17489, Germany

Eggert Stockfleth

Charité-Universitätsmedizin Berlin, University Medical School, Department of Dermatology, Venerology and Allergology and Skin Cancer Center, Berlin, 10117, Germany

Jürgen Lademann

Charité-Universitätsmedizin Berlin, University Medical School, Department of Dermatology, Venerology and Allergology and Center of Experimental and Applied Skin Physiology, Berlin, 10117, Germany

J. Biomed. Opt. 14(5), 054001 (September 08, 2009). doi:10.1117/1.3213603
History: Received March 12, 2009; Revised June 19, 2009; Accepted July 06, 2009; Published September 08, 2009
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Epidermal wound healing is a complex and dynamic regenerative process necessary to reestablish skin integrity. Fluorescence confocal laser scanning microscopy (FLSM) is a noninvasive imaging technique that has previously been used for evaluation of inflammatory and neoplastic skin disorders in vivo and at high resolution. We employed FLSM to investigate the evolution of epidermal wound healing noninvasively over time and in vivo. Two suction blisters were induced on the volar forearms of the study participants, followed by removal of the epidermis. To study the impact of wound ointment on the process of reepithelization, test sites were divided into two groups, of which one test site was left untreated as a negative control. FLSM was used for serial/consecutive evaluations up to 8days. FLSM was able to visualize the development of thin keratinocyte layers developing near the wound edge and around hair follicles until the entire epidermis has been reestablished. Wounds treated with the wound ointment were found to heal significantly faster than untreated wounds. This technique allows monitoring of the kinetics of wound healing noninvasively and over time, while offering new insights into the potential effects of topically applied drugs on the process of tissue repair.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Bernhard Lange-Asschenfeldt ; Alena Alborova ; Daniela Krüger-Corcoran ; Alexa Patzelt ; Heike Richter, et al.
"Effects of a topically applied wound ointment on epidermal wound healing studied by in vivo fluorescence laser scanning microscopy analysis", J. Biomed. Opt. 14(5), 054001 (September 08, 2009). ; http://dx.doi.org/10.1117/1.3213603


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