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Research Papers

Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage

[+] Author Affiliations
Wen Lo

National Taiwan University, Taipei, Taiwan and National Cheng Kung University, Department of Engineering Science, Tainan, Taiwan

Yu-Lin Chang, Jia-Shiu Liu, Chiu-Mei Hseuh, Vladimir Hovhannisyan

National Taiwan University, Department of Physics and Center for Quantum Science and Engineering, Taipei, Taiwan

Shean-Jen Chen

National Cheng Kung University, Department of Engineering Science, Tainan, Taiwan

Hsin-Yuan Tan

Chang Gung University, College of Medicine, Chang Gung Memorial Hospital, Department of Ophthalmology, Tao Yuan 333, Taiwan and National Taiwan University, College of Medicine and College of Engineering, Institute of Biomedical Engineering, Taipei, Taiwan

Chen-Yuan Dong

National Taiwan University, Department of Physics and Center for Quantum Science and Engineering, Taipei, Taiwan

J. Biomed. Opt. 14(5), 054003 (July 09, 2008April 22, 2009July 06, 2009October 01, 2009). doi:10.1117/1.3213602
History: Received July 09, 2008; Revised April 22, 2009; Accepted July 06, 2009; Published October 01, 2009
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We used the combination of multiphoton autofluorescence (MAF), forward second-harmonic generation (FWSHG), and backward second-harmonic generation (BWSHG) imaging for the qualitative and quantitative characterization of thermal damage of ex vivo bovine cornea. We attempt to characterize the structural alterations by qualitative MAF, FWSHG, and BWSHG imaging in the temperature range of 37to90°C. In addition to measuring the absolute changes in the three types of signals at the stromal surface, we also performed image correlation analysis between FWSHG and BWSHG and demonstrate that with increasing thermal damage, image correlation between FWSHG and BWSHG significantly increases. Our results show that while MAF and BWSHG intensities may be used as preliminary indicators of the extent of corneal thermal damage, the most sensitive measures are provided by the decay in FWSHG intensity and the convergence of FWSHG and BWSHG images.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Wen Lo ; Yu-Lin Chang ; Shean-Jen Chen ; Hsin-Yuan Tan ; Chen-Yuan Dong, et al.
"Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage", J. Biomed. Opt. 14(5), 054003 (July 09, 2008April 22, 2009July 06, 2009October 01, 2009). ; http://dx.doi.org/10.1117/1.3213602


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