Research Papers

Cyanine-loaded lipid nanoparticles for improved in vivo fluorescence imaging

[+] Author Affiliations
Isabelle Texier, Mathieu Goutayer, Anabela Da Silva, Laurent Guyon, Nadia Djaker

Commissariat à l’Énergie Atomique, LETI-DTBS, 17 rue des Martyrs, Grenoble Cedex, 38054, France

Véronique Josserand

Institut Albert Bonniot, INSERM U823, La Tronche, 38706, France

Emmanuelle Neumann

IBS J.-P. Ebel, UMR 5075 CNRS-CEA-UJF, 41 rue Jules Horowitz, Grenoble Cedex 1, 38027, France

Jérôme Bibette

Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris, Laboratoire des colloïdes et matériaux divisés, 10 rue Vauquelin, Paris Cedex 5, 75231, France

Françoise Vinet

Commissariat à l’Énergie Atomique, LETI-DTBS, 17 rue des Martyrs, Grenoble Cedex, 38054, France

J. Biomed. Opt. 14(5), 054005 (September 08, 2009). doi:10.1117/1.3213606
History: Received March 30, 2009; Revised June 30, 2009; Accepted July 07, 2009; Published September 08, 2009
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Fluorescence is a very promising radioactive-free technique for functional imaging in small animals and, in the future, in humans. However, most commercial near-infrared dyes display poor optical properties, such as low fluorescence quantum yields and short fluorescence lifetimes. In this paper, we explore whether the encapsulation of infrared cyanine dyes within the core of lipid nanoparticles (LNPs) could improve their optical properties. Lipophilic dialkylcarbocyanines DiD and DiR are loaded very efficiently in 3035-nm-diam lipid droplets stabilized in water by surfactants. No significant fluorescence autoquenching is observed up to 53 dyes per particle. Encapsulated in LNP, which are stable for more than one year at room temperature in HBS buffer (HEPES 0.02 M, EDTA 0.01 M, pH 5.5), DiD and DiR display far improved fluorescence quantum yields Φ (respectively, 0.38 and 0.25) and longer fluorescence lifetimes τ (respectively, 1.8 and 1.1ns) in comparison to their hydrophilic counterparts Cy5 (Φ=0.28, τ=1.0ns) and Cy7 (Φ=0.13, τ=0.57ns). Moreover, dye-loaded LNPs are able to accumulate passively in various subcutaneous tumors in mice, thanks to the enhanced permeability and retention effect. These new fluorescent nanoparticles therefore appear as very promising labels for in vivo fluorescence imaging.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Isabelle Texier ; Mathieu Goutayer ; Anabela Da Silva ; Laurent Guyon ; Nadia Djaker, et al.
"Cyanine-loaded lipid nanoparticles for improved in vivo fluorescence imaging", J. Biomed. Opt. 14(5), 054005 (September 08, 2009). ; http://dx.doi.org/10.1117/1.3213606


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