Research Papers

Profile of new green fluorescent protein transgenic Jinhua pigs as an imaging source

[+] Author Affiliations
Tatsuo Kawarasaki

Shizuoka Prefectural Livestock Institute, Swine and Poultry Research Center, 2780 Nishikata, Kikugawa, Shizuoka 439-0037, Japan

Kazuhiko Uchiyama

Kitasato University School of Medicine, Department of Laboratory Animal Science, Sagamihara, Kanagawa 228-8555, Japan

Atsushi Hirao

Jichi Medical University, Division of Anatomy, Department of Anatomy, Tochigi 329-0498, Japan

Sadahiro Azuma

Kitasato University School of Medicine, Department of Laboratory Animal Science, Sagamihara, Kanagawa 228-8555, Japan

Masayoshi Otake, Masatoshi Shibata, Seiko Tsuchiya

Shizuoka Prefectural Livestock Institute, Swine and Poultry Research Center, 2780 Nishikata, Kikugawa, Shizuoka 439-0037, Japan

Shin Enosawa

National Research Institute for Child Health and Development, Department of Innovative Surgery, Tokyo 157-8535, Japan

Koichi Takeuchi

Jichi Medical University, Division of Anatomy, Department of Anatomy, Tochigi 329-0498, Japan

Kenjiro Konno, Yoji Hakamata

Jichi Medical University, Center for Experimental Medicine, Tochigi 329-0498, Japan

Hiroyuki Yoshino, Takuya Wakai

Jichi Medical University, Center for Molecular Medicine, Division of Organ Replacement Research, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Tochigi, 329-0498, Japan

Shigeo Ookawara

Jichi Medical University, Division of Anatomy, Department of Anatomy, Tochigi 329-0498, Japan

Hozumi Tanaka

Jichi Medical University, Center for Experimental Medicine, Tochigi 329-0498, Japan

Eiji Kobayashi, Takashi Murakami

Jichi Medical University, Center for Molecular Medicine, Division of Organ Replacement Research, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan

J. Biomed. Opt. 14(5), 054017 (October 12, 2009). doi:10.1117/1.3241985
History: Received November 20, 2008; Revised July 26, 2009; Accepted July 28, 2009; Published October 12, 2009
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Animal imaging sources have become an indispensable material for biological sciences. Specifically, gene-encoded biological probes serve as stable and high-performance tools to visualize cellular fate in living animals. We use a somatic cell cloning technique to create new green fluorescent protein (GFP)-expressing Jinhua pigs with a miniature body size, and characterized the expression profile in various tissues/organs and ex vivo culture conditions. The born GFP-transgenic pig demonstrate an organ/tissue-dependent expression pattern. Strong GFP expression is observed in the skeletal muscle, pancreas, heart, and kidney. Regarding cellular levels, bone-marrow-derived mesenchymal stromal cells, hepatocytes, and islet cells of the pancreas also show sufficient expression with the unique pattern. Moreover, the cloned pigs demonstrate normal growth and fertility, and the introduced GFP gene is stably transmitted to pigs in subsequent generations. The new GFP-expressing Jinhua pigs may be used as new cellular/tissue light resources for biological imaging in preclinical research fields such as tissue engineering, experimental regenerative medicine, and transplantation.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Tatsuo Kawarasaki ; Kazuhiko Uchiyama ; Atsushi Hirao ; Sadahiro Azuma ; Masayoshi Otake, et al.
"Profile of new green fluorescent protein transgenic Jinhua pigs as an imaging source", J. Biomed. Opt. 14(5), 054017 (October 12, 2009). ; http://dx.doi.org/10.1117/1.3241985


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