0
Research Papers

Influence of laser parameters on nanoparticle-induced membrane permeabilization

[+] Author Affiliations
Cuiping Yao

Ministry of Education, Key Laboratory of Biomedical Information Engineering, China and Xi’an Jiaotong University, School of Life Science and Technology, Institute of Biomedical Analytical Technology and Instrumentation, Xianning xi Road 28, Xi’an, 710049, China

Xiaochao Qu

Xidian University, School of Electronic Engineering, Life Science Research Center, Xi’an, Shaanxi 710071, China

Zhenxi Zhang

Ministry of Education, Key Laboratory of Biomedical Information Engineering, China and Xi’an Jiaotong University, School of Life Science and Technology, Institute of Biomedical Analytical Technology and Instrumentation, Xianning xi Road 28, Xi’an, 710049, China

Gereon Hüttmann, Ramtin Rahmanzadeh

University of Lübeck, Institute of Biomedical Optics, Peter-Monnik-Weg 4, Lübeck, D-23562, Germany

J. Biomed. Opt. 14(5), 054034 (October 27, 2009). doi:10.1117/1.3253320
History: Received October 09, 2008; Revised August 06, 2009; Accepted August 12, 2009; Published October 27, 2009
Text Size: A A A

Light-absorbing nanoparticles that are heated by short laser pulses can transiently increase membrane permeability. We evaluate the membrane permeability by flow cytometry assaying of propidium iodide and fluorescein isothiocyanate dextran (FITC-D) using different laser sources. The dependence of the transfection efficiency on laser parameters such as pulse duration, irradiant exposure, and irradiation mode is investigated. For nano- and also picosecond irradiation, we show a parameter range where a reliable membrane permeabilization is achieved for 10-kDa FITC-D. Fluorescent labeled antibodies are able to penetrate living cells that are permeabilized using these parameters. More than 50% of the cells are stained positive for a 150-kDa IgG antibody. These results suggest that the laser-induced permeabilization approach constitutes a promising tool for targeted delivery of larger exogenous molecules into living cells.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Cuiping Yao ; Xiaochao Qu ; Zhenxi Zhang ; Gereon Hüttmann and Ramtin Rahmanzadeh
"Influence of laser parameters on nanoparticle-induced membrane permeabilization", J. Biomed. Opt. 14(5), 054034 (October 27, 2009). ; http://dx.doi.org/10.1117/1.3253320


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
 
Your Session has timed out. Please sign back in to continue.
Sign In to Access Full Content

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


Buy this article ($18 for members, $25 for non-members).
Sign In