Research Papers

Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo

[+] Author Affiliations
Junjie Yao, Konstantin Maslov, Song Hu, Lihong V. Wang

Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130

J. Biomed. Opt. 14(5), 054049 (October 26, 2009). doi:10.1117/1.3251044
History: Received May 09, 2009; Revised August 28, 2009; Accepted September 02, 2009; Published October 26, 2009
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Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries results in discontinuous and fragmentary capillary images. To overcome this problem, we use Evans blue (EB) dye as a contrast agent for in vivo photoacoustic imaging. EB has strong optical absorption and distributes uniformly in the blood stream by chemically binding to albumin. With the help of EB, complete and continuous microvascular networks—especially capillaries—are imaged. The diffusion dynamics of EB leaving the blood stream and the clearance dynamics of the EB-albumin complex are also quantitatively investigated.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Junjie Yao ; Konstantin Maslov ; Song Hu and Lihong V. Wang
"Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo", J. Biomed. Opt. 14(5), 054049 (October 26, 2009). ; http://dx.doi.org/10.1117/1.3251044


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