Research Papers

Imaging of goblet cells as a marker for intestinal metaplasia of the stomach by one-photon and two-photon fluorescence endomicroscopy

[+] Author Affiliations
Hongchun Bao

Swinburne University of Technology, Center for Micro-Photonics, Faculty of Engineering & Industrial Sciences, P.O. Box 218 John Street, Hawthorn, Victoria 3122, Australia

Alex Boussioutas

Research-Peter MacCallum Cancer Centre, 1 St. Andrews Place, East Melbourne, Victoria 3002, Australia and University of Melbourne, Western Hospital, Department of Medicine (RMH/WH), Footscray, 3011, Australia

Jeremy Reynolds

Research-Peter MacCallum Cancer Centre, 1 St. Andrews Place, East Melbourne, Victoria 3002, Australia

Sarah Russell

Swinburne University of Technology, Center for Micro-Photonics, Faculty of Engineering & Industrial Sciences, P.O. Box 218 John Street, Hawthorn, Victoria 3122, Australia and Research-Peter MacCallum Cancer Centre, 1 St. Andrews Place, East Melbourne, Victoria 3002, Australia

Min Gu

Swinburne University of Technology, Center for Micro-Photonics, Faculty of Engineering & Industrial Sciences, P.O. Box 218 John Street, Hawthorn, Victoria 3122, Australia

J. Biomed. Opt. 14(6), 064031 (December 07, 2009). doi:10.1117/1.3269681
History: Received May 03, 2009; Revised October 01, 2009; Accepted October 13, 2009; Published December 07, 2009; Online December 07, 2009
Text Size: A A A

Abstract

Goblet cells are a requirement for the diagnosis of intestinal metaplasia of the stomach. The gastric mucosa is lined by a monolayer of columnar epithelium with some specialization at the crypts, but there are no goblet cells in normal gastric epithelium. The appearance of goblet cells in gastric epithelium is an indicator of potential malignant progression toward adenocarcinoma. Therefore, in vivo three-dimensional imaging of goblet cells is essential for diagnoses of a premalignant stage of gastric cancers called intestinal metaplasia. We used mouse intestine, which has goblet cells, as a model of intestinal metaplasia. One-photon confocal fluorescence endomicroscopy and two-photon fluorescence endomicroscopy are employed for 3-D imaging of goblet cells. The penetration depth, the sectioning ability, and the photobleaching information of imaging are demonstrated. Both endomicroscopy techniques can three-dimensionally observe goblet cells in mouse large intestine and achieve an imaging depth of 176μm. The two-photon fluorescence endomicroscopy shows higher resolution and contrast of the imaging sections at each depth. In addition, two-photon fluorescence endomicroscopy also has advantages of sectioning ability and less photobleaching. These results prove that two-photon fluorescence endomicroscopy is advantageous in diagnoses of a premalignant stage of gastric cancers.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Hongchun Bao ; Alex Boussioutas ; Jeremy Reynolds ; Sarah Russell and Min Gu
"Imaging of goblet cells as a marker for intestinal metaplasia of the stomach by one-photon and two-photon fluorescence endomicroscopy", J. Biomed. Opt. 14(6), 064031 (December 07, 2009). ; http://dx.doi.org/10.1117/1.3269681


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.