Research Papers

Is there a difference between T- and B-lymphocyte morphology?

[+] Author Affiliations
Dmitry I. Strokotov, Maxim A. Yurkin, Konstantin V. Gilev

Institute of Chemical Kinetics and Combustion, Siberian Branch RAS, Institutskaya 3, Novosibirsk, 630090, Russia and Novosibirsk State University, Pirogova 2, Novosibirsk, 630090, Russia

Dirk R. van Bockstaele

Esoterix, Clinical Trials Services, Division of LabCorp, Gen. De Wittelaan L11-A2, Mechelen, B-2800, Belgium

Alfons G. Hoekstra

University of Amsterdam, Faculty of Science, Science Park 107, Amsterdam, 1098 XG, Netherlands

Nikolay B. Rubtsov

Institute of Cytology and Genetics, Siberian Branch RAS, Lavrentyeva 10, Novosibirsk, 630090, Russia

Valeri P. Maltsev

Institute of Chemical Kinetics and Combustion, Siberian Branch RAS, Institutskaya 3, Novosibirsk, 630090, Russia and Novosibirsk State University, Pirogova 2, Novosibirsk, 630090, Russia

J. Biomed. Opt. 14(6), 064036 (December 29, 2009). doi:10.1117/1.3275471
History: Received June 09, 2009; Revised October 15, 2009; Accepted October 19, 2009; Published December 29, 2009; Online December 29, 2009
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We characterize T- and B-lymphocytes from several donors, determining cell diameter, ratio of nucleus to cell diameter, and refractive index of the nucleus and cytoplasm for each individual cell. We measure light-scattering profiles with a scanning flow cytometer and invert the signals using a coated sphere as an optical model of the cell and by relying on a global optimization technique. The main difference in morphology of T- and B-lymphocytes is found to be the larger mean diameters of the latter. However, the difference is smaller than the natural biological variability of a single cell. We propose nuclear inhomogeneity as a possible reason for the deviation of measured light-scattering profiles from real lymphocytes from those obtained from the coated sphere model.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Dmitry I. Strokotov ; Maxim A. Yurkin ; Konstantin V. Gilev ; Dirk R. van Bockstaele ; Alfons G. Hoekstra, et al.
"Is there a difference between T- and B-lymphocyte morphology?", J. Biomed. Opt. 14(6), 064036 (December 29, 2009). ; http://dx.doi.org/10.1117/1.3275471


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