Research Papers

Improved three-dimensional Fourier domain optical coherence tomography by index matching in alveolar structures

[+] Author Affiliations
Sven Meissner

University of Technology Dresden, Faculty of Medicine Carl Gustav Carus, Clinical Sensoring and Monitoring, Fetscherstrasse 74, Dresden, 01307, Germany

Lilla Knels

University of Technology Dresden, Department of Anaesthesiology and Intensive Care, Faculty of Medicine Carl Gustav Carus, Fetscherstrasse 74, Dresden, 01307, Germany

Edmund Koch

University of Technology Dresden, Faculty of Medicine Carl Gustav Carus, Clinical Sensoring and Monitoring, Fetscherstrasse 74, Dresden, 01307, Germany

J. Biomed. Opt. 14(6), 064037 (December 21, 2009). doi:10.1117/1.3275472
History: Received June 10, 2009; Revised October 13, 2009; Accepted October 20, 2009; Published December 21, 2009; Online December 21, 2009
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Three-dimensional Fourier domain optical coherence tomography (3-D FDOCT) is used to demonstrate that perfusion fixation with a mixture of glutaraldehyde and paraformaldehyde does not alter the geometry of subpleural lung parenchyma in isolated and perfused rabbit lungs. This is confirmed by simultaneous imaging of lung parenchyma with intravital microscopy. To eliminate the diffraction index interfaces between alveolar pockets and walls, we fill the fixed lungs with ethanol by perfusing with gradually increasing concentrations. This bottom-up filling process leaves no remaining air bubbles in the alveolar structures, thus drastically improving the resolution and penetration depth of 3-D FDOCT imaging. We observe an approximately 18% increase in alveolar area after ethanol filling, likely due in large part to elimination of the air/tissue interfaces. 3-D OCT datasets acquired from ethanol-filled lungs allow segmentation of the ethanol-filled structures, which were formerly air-filled, and 3-D reconstruction of larger areas of subpleural alveolar structures. Our innovative process of filling the lungs with ethanol postperfusion fixation thus enables more accurate quantification of alveolar geometries, a critical component of modeling lung function.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Sven Meissner ; Lilla Knels and Edmund Koch
"Improved three-dimensional Fourier domain optical coherence tomography by index matching in alveolar structures", J. Biomed. Opt. 14(6), 064037 (December 21, 2009). ; http://dx.doi.org/10.1117/1.3275472


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