0
Special Section on Optical Methods in Vascular Biology and Medicine

In vivo high-resolution structural imaging of large arteries in small rodents using two-photon laser scanning microscopy

[+] Author Affiliations
Mirjam G. A. oude Egbrink

Maastricht University, Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht, 6200 MD, The Netherlands

Wim Engels

Maastricht University, Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht, Maastricht, 6200 MD, The Netherlands

Peter J. A. Leenders

Maastricht University, Pharmacology and Toxicology, Cardiovascular Research Institute Maastricht, Maastricht, 6200 MD, The Netherlands

Ellen J. L. Brunenberg

University of Technology, Department of Biomedical Engineering, WH 1.105, P.O. Box 513, Eindhoven, 5600 MB, The Netherlands

Koen D. Reesink

Maastricht University, Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht, Maastricht, 6200 MD, The Netherlands

Ben J. A. Janssen

Maastricht University, Pharmacology and Toxicology, Cardiovascular Research Institute Maastricht, Maasticht, 6200 MD, The Netherlands

Bart M. ter Haar Romeny

University of Technology, Department of Biomedical Engineering, WH 1.105, P.O. Box 513, Eindhoven, 5600 MB, The Netherlands

Dick W. Slaaf

Maastricht University, Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht, Maastricht, 6200 MD, The Netherlands and University of Technology, Department of Biomedical Engineering, WH 1.105, P.O. Box 513, Eindhoven, 5600 MB, The Netherlands

Marc A. M. J. van Zandvoort

Maastricht University, Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht, Maastricht, 6200 MD, The Netherlands

Remco T. A. Megens, Sietze Reitsma, Lenneke Prinzen

Maastricht University, Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht, Maastricht, 6200 MD, The Netherlands

J. Biomed. Opt. 15(1), 011108 (January 25, 2010). doi:10.1117/1.3281672
History: Received April 04, 2009; Revised August 14, 2009; Accepted September 01, 2009; Published January 25, 2010; Online January 25, 2010
Text Size: A A A

In vivo (molecular) imaging of the vessel wall of large arteries at subcellular resolution is crucial for unraveling vascular pathophysiology. We previously showed the applicability of two-photon laser scanning microscopy (TPLSM) in mounted arteries ex vivo. However, in vivo TPLSM has thus far suffered from in-frame and between-frame motion artifacts due to arterial movement with cardiac and respiratory activity. Now, motion artifacts are suppressed by accelerated image acquisition triggered on cardiac and respiratory activity. In vivo TPLSM is performed on rat renal and mouse carotid arteries, both surgically exposed and labeled fluorescently (cell nuclei, elastin, and collagen). The use of short acquisition times consistently limit in-frame motion artifacts. Additionally, triggered imaging reduces between-frame artifacts. Indeed, structures in the vessel wall (cell nuclei, elastic laminae) can be imaged at subcellular resolution. In mechanically damaged carotid arteries, even the subendothelial collagen sheet (1μm) is visualized using collagen-targeted quantum dots. We demonstrate stable in vivo imaging of large arteries at subcellular resolution using TPLSM triggered on cardiac and respiratory cycles. This creates great opportunities for studying (diseased) arteries in vivo or immediate validation of in vivo molecular imaging techniques such as magnetic resonance imaging (MRI), ultrasound, and positron emission tomography (PET).

Figures in this Article
© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Mirjam G. A. oude Egbrink ; Wim Engels ; Peter J. A. Leenders ; Ellen J. L. Brunenberg ; Koen D. Reesink, et al.
"In vivo high-resolution structural imaging of large arteries in small rodents using two-photon laser scanning microscopy", J. Biomed. Opt. 15(1), 011108 (January 25, 2010). ; http://dx.doi.org/10.1117/1.3281672


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
 
Your Session has timed out. Please sign back in to continue.
Sign In to Access Full Content

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement

Buy this article ($18 for members, $25 for non-members).
Sign In