Research Papers: General

Use of optical tweezers to probe epithelial mechanosensation

[+] Author Affiliations
Andrew Resnick

Cleveland State University, Department of Physics, 2121 Euclid Avenue, Cleveland, Ohio 44115

J. Biomed. Opt. 15(1), 015005 (February 18, 2010). doi:10.1117/1.3316378
History: Received July 17, 2009; Revised November 11, 2009; Accepted December 23, 2009; Published February 18, 2010; Online February 18, 2010
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Cellular mechanosensation mechanisms have been implicated in a variety of disease states. Specifically in renal tubules, the primary cilium and associated mechanosensitive ion channels are hypothesized to play a role in water and salt homeostasis, with relevant disease states including polycystic kidney disease and hypertension. Previous experiments investigating ciliary-mediated cellular mechanosensation have used either fluid flow chambers or micropipetting to elicit a biological response. The interpretation of these experiments in terms of the “ciliary hypothesis” has been difficult due the spatially distributed nature of the mechanical disturbance—several competing hypotheses regarding possible roles of primary cilium, glycocalyx, microvilli, cell junctions, and actin cytoskeleton exist. I report initial data using optical tweezers to manipulate individual primary cilia in an attempt to elicit a mechanotransduction response—specifically, the release of intracellular calcium. The advantage of using laser tweezers over previous work is that the applied disturbance is highly localized. I find that stimulation of a primary cilium elicits a response, while stimulation of the apical surface membrane does not. These results lend support to the hypothesis that the primary cilium mediates transduction of mechanical strain into a biochemical response in renal epithelia.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Andrew Resnick
"Use of optical tweezers to probe epithelial mechanosensation", J. Biomed. Opt. 15(1), 015005 (February 18, 2010). ; http://dx.doi.org/10.1117/1.3316378


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