Research Papers: Imaging

Fluorescence imaging of vascular endothelial growth factor in tumors for mice embedded in a turbid medium

[+] Author Affiliations
Nrusingh C. Biswal, John K. Gamelin

University of Connecticut, Department of Electrical and Computer Engineering, 371 Fairfield Road U2157, Storrs, Connecticut 06269

Baohong Yuan

Catholic University of America, Department of Biomedical Engineering, 620 Michigan Avenue North East, Washington DC 20064

Marina V. Backer, Joseph M. Backer

SibTech, Inc., 115A Commerce Drive, Brookfield, Connecticut 06804

Quing Zhu

University of Connecticut, Department of Electrical and Computer Engineering, 371 Fairfield Road U2157, Storrs, Connecticut 06269

J. Biomed. Opt. 15(1), 016012 (February 18, 2010). doi:10.1117/1.3306704
History: Received March 22, 2009; Revised November 23, 2009; Accepted November 24, 2009; Published February 18, 2010; Online February 18, 2010
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We demonstrate the feasibility of fluorescence imaging of deeply seated tumors using mice injected with an angiogenesis tracer, a vascular endothelial growth factor conjugated with the infrared dye cyanine 7 (VEGF/Cy7). Our optical-only imaging reconstruction method separately estimates the target depth, and then applies this information to reconstruct functional information such as fluorophore concentration. Fluorescence targets with concentrations as low as sub-25nM are well reconstructed at depths up to 2cm in both homogeneous and heterogeneous media with this technique.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Nrusingh C. Biswal ; John K. Gamelin ; Baohong Yuan ; Marina V. Backer ; Joseph M. Backer, et al.
"Fluorescence imaging of vascular endothelial growth factor in tumors for mice embedded in a turbid medium", J. Biomed. Opt. 15(1), 016012 (February 18, 2010). ; http://dx.doi.org/10.1117/1.3306704


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