Research Papers: Sensing

In vivo native fluorescence spectroscopy and nicotinamide adinine dinucleotide/flavin adenine dinucleotide reduction and oxidation states of oral submucous fibrosis for chemopreventive drug monitoring

[+] Author Affiliations
Shanmugam Sivabalan

Anna University Chennai, Department of Physics, Division of Medical Physics and Lasers, Chennai, 600 025 India

C. Ponranjini Vedeswari, Sadaksharam Jayachandran

Tamilnadu Government Dental College and Hospital, Department of Oral Medicine and Radiology, Chennai, 600 003 India

Dornadula Koteeswaran, Chidambaranathan Pravda

Meenakshi Ammal Dental College and Hospital, Department of Oral Medicine and Radiology, Chennai, 600 095 India

Prakasa Rao Aruna, Singaravelu Ganesan

Anna University Chennai, Department of Physics, Division of Medical Physics and Lasers, Chennai, 600 025 India

J. Biomed. Opt. 15(1), 017010 (March 02, 2010). doi:10.1117/1.3324771
History: Received May 10, 2009; Revised January 03, 2010; Accepted January 05, 2010; Published March 02, 2010; Online March 02, 2010
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Native fluorescence spectroscopy has shown potential to characterize and diagnose oral malignancy. We aim at extending the native fluorescence spectroscopy technique to characterize normal and oral submucous fibrosis (OSF) patients under pre- and post-treated conditions, and verify whether this method could also be considered in the monitoring of therapeutic prognosis noninvasively. In this study, 28 normal subjects and 28 clinically proven cases of OSF in the age group of 20to40years are diagnosed using native fluorescence spectroscopy. The OSF patients are given dexamethasone sodium phosphate and hyaluronidase twice a week for 6weeks, and the therapeutic response is monitored using fluorescence spectroscopy. The fluorescence emission spectra of normal and OSF cases of both pre- and post-treated conditions are recorded in the wavelength region of 350to600nm at an excitation wavelength of 330nm. The statistical significance is verified using discriminant analysis. The oxidation-reduction ratio of the tissue is also calculated using the fluorescence emission intensities of flavin adenine dinucleotide and nicotinamide adinine dinucleotide at 530 and 440nm, respectively, and they are compared with conventional physical clinical examinations. This study suggests that native fluorescence spectroscopy could also be extended to OSF diagnosis and therapeutic prognosis.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Shanmugam Sivabalan ; C. Ponranjini Vedeswari ; Sadaksharam Jayachandran ; Dornadula Koteeswaran ; Chidambaranathan Pravda, et al.
"In vivo native fluorescence spectroscopy and nicotinamide adinine dinucleotide/flavin adenine dinucleotide reduction and oxidation states of oral submucous fibrosis for chemopreventive drug monitoring", J. Biomed. Opt. 15(1), 017010 (March 02, 2010). ; http://dx.doi.org/10.1117/1.3324771


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