Research Papers: Therapeutic

Preferential accumulation of 5-aminolevulinic acid-induced protoporphyrin IX in breast cancer: a comprehensive study on six breast cell lines with varying phenotypes

[+] Author Affiliations
Stacy R. Millon

Duke University, Department of Biomedical Engineering, 3000 Science Drive Hudson Hall, Box 90281, Durham, North Carolina 27708-0281

Julie H. Ostrander

Duke University, Department of Medicine, Box 2628 Medical Center, Durham, North Carolina 27710

Siavash Yazdanfar

General Electric Global Research Center, 1 Research Circle, Niskayuna, New York 12309

J. Quincy Brown, Janelle E. Bender, Anita Rajeha, Nirmala Ramanujam

Duke University, Department of Biomedical Engineering, 3000 Science Drive Hudson Hall, Box 90281, Durham, North Carolina 27708-0281

J. Biomed. Opt. 15(1), 018002 (February 18, 2010). doi:10.1117/1.3302811
History: Received April 13, 2009; Revised October 26, 2009; Accepted November 30, 2009; Published February 18, 2010; Online February 18, 2010
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We describe the potential of 5-aminolevulinic acid (ALA)–induced protoporphyrin IX (PpIX) fluorescence as a source of contrast for margin detection in commonly diagnosed breast cancer subtypes. Fluorescence intensity of PpIX in untreated and ALA-treated normal mammary epithelial and breast cancer cell lines of varying estrogen receptor expression were quantitatively imaged with confocal micro-scopy. Percentage change in fluorescence intensity integrated over 610700nm (attributed to PpIX) of posttreated compared to pretreated cells showed statistically significant differences between four breast cancer and two normal mammary epithelial cell lines. However, a direct comparison of post-treatment PpIX fluorescence intensities showed no differences between breast cancer and normal mammary epithelial cell lines due to confounding effects by endogenous fluorescence from flavin adenine dinucleotide (FAD). Clinically, it is impractical to obtain pre- and post-treatment images. Thus, spectral imaging was demonstrated as a means to remove the effects of endo-genous FAD fluorescence allowing for discrimination between post-treatment PpIX fluorescence of four breast cancer and two normal mammary epithelial cell lines. Fluorescence spectral imaging of ALA-treated breast cancer cells showed preferential PpIX accumulation regardless of malignant phenotype and suggests a useful contrast mechanism for discrimination of residual cancer at the surface of breast tumor margins.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Stacy R. Millon ; Julie H. Ostrander ; Siavash Yazdanfar ; J. Quincy Brown ; Janelle E. Bender, et al.
"Preferential accumulation of 5-aminolevulinic acid-induced protoporphyrin IX in breast cancer: a comprehensive study on six breast cell lines with varying phenotypes", J. Biomed. Opt. 15(1), 018002 (February 18, 2010). ; http://dx.doi.org/10.1117/1.3302811


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