Research Papers: Imaging

Wavefront optimized nonlinear microscopy of ex vivo human retinas

[+] Author Affiliations
Emilio J. Gualda, Juan M. Bueno, Pablo Artal

Universidad de Murcia, Laboratorio de Óptica, Campus de Espinardo (CiOyN), Murcia, 30100 Spain

J. Biomed. Opt. 15(2), 026007 (March 29, 2010). doi:10.1117/1.3369001
History: Received September 21, 2009; Revised January 20, 2010; Accepted January 22, 2010; Published March 29, 2010; Online March 29, 2010
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A multiphoton microscope incorporating a Hartmann-Shack (HS) wavefront sensor to control the ultrafast laser beam’s wavefront aberrations has been developed. This instrument allowed us to investigate the impact of the laser beam aberrations on two-photon autofluorescence imaging of human retinal tissues. We demonstrated that nonlinear microscopy images are improved when laser beam aberrations are minimized by realigning the laser system cavity while wavefront controlling. Nonlinear signals from several human retinal anatomical features have been detected for the first time, without the need of fixation or staining procedures. Beyond the improved image quality, this approach reduces the required excitation power levels, minimizing the side effects of phototoxicity within the imaged sample. In particular, this may be important to study the physiology and function of the healthy and diseased retina.

Figures in this Article
Mira 900f, Coherent, Santa Clara, California

Citation

Emilio J. Gualda ; Juan M. Bueno and Pablo Artal
"Wavefront optimized nonlinear microscopy of ex vivo human retinas", J. Biomed. Opt. 15(2), 026007 (March 29, 2010). ; http://dx.doi.org/10.1117/1.3369001


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