Research Papers: Imaging

Nonlinear multicontrast microscopy of hematoxylin-and-eosin-stained histological sections

[+] Author Affiliations
Adam Tuer, Danielle Tokarz, Nicole Prent, Richard Cisek

University of Toronto, Department of Physics, Institute for Optical Sciences, Department of Chemical and Physical Sciences, 3359 Mississauga Road North, Mississauga, Ontario, L5L 1C6 Canada

Jennifer Alami, Daniel J. Dumont, Ludmila Bakueva, John Rowlands

University of Toronto, Sunnybrook Health Sciences Centre, Department of Medical Biophysics, 610 University Avenue, Toronto, Ontario M5G 2M9, Canada

Virginijus Barzda

University of Toronto, Department of Physics, Institute for Optical Sciences, Department of Chemical and Physical Sciences, 3359 Mississauga Road North, Mississauga, Ontario, L5L 1C6 Canada

J. Biomed. Opt. 15(2), 026018 (April 28, 2010). doi:10.1117/1.3382908
History: Received September 10, 2009; Revised February 05, 2010; Accepted February 22, 2010; Published April 28, 2010; Online April 28, 2010
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Imaging hematoxylin-and-eosin-stained cancerous histological sections with multicontrast nonlinear excitation fluorescence, second- and third-harmonic generation (THG) microscopy reveals cellular structures with extremely high image contrast. Absorption and fluorescence spectroscopy together with second hyperpolarizability measurements of the dyes shows that strong THG appears due to neutral hemalum aggregation and is subsequently enhanced by interaction with eosin. Additionally, fluorescence lifetime imaging microscopy reveals eosin fluorescence quenching by hemalums, showing better suitability of only eosin staining for fluorescence microscopy. Multicontrast nonlinear microscopy has the potential to differentiate between cancerous and healthy tissue at a single cell level.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Adam Tuer ; Danielle Tokarz ; Nicole Prent ; Richard Cisek ; Jennifer Alami, et al.
"Nonlinear multicontrast microscopy of hematoxylin-and-eosin-stained histological sections", J. Biomed. Opt. 15(2), 026018 (April 28, 2010). ; http://dx.doi.org/10.1117/1.3382908


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