Research Papers: Imaging

Multispectral in vivo three-dimensional optical coherence tomography of human skin

[+] Author Affiliations
Aneesh Alex

Cardiff University, School of Optometry and Vision Sciences, Biomedical Imaging Group, Cardiff, Wales, United Kingdom

Boris Považay, Bernd Hofer

Medical University Vienna, Center for Medical Physics and Biomedical Engineering, Währinger Gürtel 18-20, Vienna, 1090 Austria and Cardiff University, School of Optometry and Vision Sciences, Biomedical Imaging Group, Cardiff, Wales, United Kingdom

Sergei Popov

Imperial College, Department of Physics, London, SW7 2AZ United Kingdom

Carl Glittenberg, Susanne Binder

Ludwig Boltzmann Institute, Department of Ophthalmology, Rudolf Foundation Clinic Vienna, Juchgasse 25, Vienna, 1030 Austria

Wolfgang Drexler

Medical University Vienna, Center for Medical Physics and Biomedical Engineering, Währinger Gürtel 18-20, Vienna, 1090 Austria and Cardiff University, School of Optometry and Vision Sciences, Biomedical Imaging Group, Cardiff, Wales, United Kingdom

J. Biomed. Opt. 15(2), 026025 (May 04, 2010). doi:10.1117/1.3400665
History: Received November 17, 2009; Revised February 16, 2010; Accepted March 04, 2010; Published May 04, 2010; Online May 04, 2010
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The capability of optical coherence tomography (OCT) to perform “optical biopsy” of tissues within a depth range of 1to2mm with micron-scale resolution in real time makes it a promising biomedical imaging modality for dermatologic applications. Three high-speed, spectrometer-based frequency-domain OCT systems operating at 800nm (20,000 A-scans/s), 1060nm, and 1300nm (both 47,000 A-scans/s) at comparable signal-to-noise ratio (SNR), SNR roll-off with scanning depth, and transverse resolution (<15μm) were used to acquire 3-D tomograms of glabrous and hairy human skin in vivo. Images obtained using these three systems were compared in terms of penetration depth, resolution, and contrast. Normal as well as abnormal sites like moles and scar tissue were examined. In this preliminary study, skin pigmentation had little effect on penetration accomplished at three different wavelengths. The epidermis and dermal–epidermal junction could be properly delineated using OCT at 800nm, and this wavelength offered better contrast over the other two wavelength regions. OCT at 1300nm permits imaging of deeper dermal layers, critical for detecting deeper tumor boundaries and other deeper skin pathologies. The performance at 1060nm compromises between the other wavelengths in terms of penetration depth and image contrast.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Aneesh Alex ; Boris Považay ; Bernd Hofer ; Sergei Popov ; Carl Glittenberg, et al.
"Multispectral in vivo three-dimensional optical coherence tomography of human skin", J. Biomed. Opt. 15(2), 026025 (May 04, 2010). ; http://dx.doi.org/10.1117/1.3400665


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