Research Papers: Imaging

Nonlinear microscopy with fiber laser continuum excitation

[+] Author Affiliations
Fredrik Svedberg, Christian Brackmann

Chalmers University of Technology, Department of Chemical and Biological Engineering, Kemivägen 10, 412 96 Göteborg, Sweden

Thomas Hellerer

TOPTICA Photonics AG, Lochhamer Schlag 19, 82166 Graefelfing (Munich), Germany

Annika Enejder

Chalmers University of Technology, Department of Chemical and Biological Engineering, Kemivägen 10, 412 96 Göteborg, Sweden

J. Biomed. Opt. 15(2), 026026 (April 06, 2010). doi:10.1117/1.3374039
History: Received January 20, 2010; Revised March 01, 2010; Accepted March 02, 2010; Published April 06, 2010; Online April 06, 2010
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Abstract

A compact high-power fiber-based femtosecond laser system is presented for coherent anti-Stokes Raman scattering/second-harmonic generation (CARS/SHG) microscopy, and quantitatively compared with a conventional picosecond optical parametric oscillator (OPO)-based system. While the broad spectral width of the femtosecond pulses results in 2.5 times lower image contrast and limited spectral selectivity, lipid stores, myosin, and collagen filaments in living cells can clearly be identified at 60 times lower excitation powers compared to the picosecond system. Visually the images contain the same information. Together with simple operation, small footprint, and low cost, the capabilities of this high-power all-fiber-based laser system promise a more general use of nonlinear microscopy within the biosciences.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Fredrik Svedberg ; Christian Brackmann ; Thomas Hellerer and Annika Enejder
"Nonlinear microscopy with fiber laser continuum excitation", J. Biomed. Opt. 15(2), 026026 (April 06, 2010). ; http://dx.doi.org/10.1117/1.3374039


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