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Research Papers: Sensing

Intraoperative delineation of primary brain tumors using time-resolved fluorescence spectroscopy

[+] Author Affiliations
Pramod V. Butte

Cedars-Sinai Medical Center, Department of Neurosurgery, 8631 West 3rd Street, Suite 800E, Los Angeles, California 90048 and University of Southern California, Department of Biomedical Engineering, 451 Health Sciences Drive, Los Angeles, California 90007

Qiyin Fang, Javier A. Jo

Cedars-Sinai Medical Center, Department of Surgery, 8700 Beverly Blvd., 8215 NT, Los Angeles, California 90048

William H. Yong

University of California, Los Angeles, Department of Pathology and Laboratory Medicine, CHS 18–161, CAMPUS-173216, Los Angeles, California 90095

Brian K. Pikul, Keith L. Black

Cedars-Sinai Medical Center, Department of Neurosurgery, 8631 West 3rd Street, Suite 800E, Los Angeles, California 90048

Laura Marcu

University of Southern California, Department of Biomedical Engineering, 451 Health Sciences Drive, Los Angeles, California 90007 and University of California, Davis, Biomedical Engineering, Genome Biomedical Science Facility,451 Health Sciences Drive, Room 2513, Davis, California 95616

J. Biomed. Opt. 15(2), 027008 (April 14, 2010). doi:10.1117/1.3374049
History: Received July 16, 2009; Revised January 18, 2010; Accepted February 09, 2010; Published April 14, 2010; Online April 14, 2010
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The goal of this study is to determine the potential of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) as an adjunctive tool for delineation of brain tumor from surrounding normal tissue in order to assist the neurosurgeon in near-complete tumor excision. A time-domain TR-LIFS prototype apparatus (gated photomultiplier detection, fast digitizer) was used for recording tissue autofluorescence in normal cortex (NC), normal white matter (NWM), and various grades of gliomas intraoperatively. Tissue fluorescence was induced with a pulsed nitrogen laser (337nm, 700ps), and the intensity decay profiles were recorded in the 360-to550-nm spectral range (10-nm interval). Histopathological analysis (hematoxylin & eosin) of the biopsy samples taken from the site of TR-LIFS measurements was used for validation of spectroscopic results. Preliminary results on 17 patients demonstrate that normal cortex (N=16) and normal white matter (N=3) show two peaks of fluorescence emission at 390nm(lifetime=1.8±0.3ns) and 460nm(lifetime=0.8±0.1ns). The 390-nm emission peak is absent in low-grade glioma (N=5; lifetime=1.1ns) and reduced in high-grade glioma (N=9; lifetime=1.7±0.4ns). The emission characteristics at 460nm in all tissues correlated with the nicotinamide adenine dinucleotide fluorescence (peak: 440to460nm; lifetime: 0.8to1.0ns). These findings demonstrate the potential of using TR-LIFS as a tool for enhanced delineation of brain tumors during surgery. In addition, this study evaluates similarities and differences between TR-LIFS signatures of brain tumors obtained in vivo and those previously reported in ex vivo brain tumor specimens.

Figures in this Article
© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Pramod V. Butte ; Qiyin Fang ; William H. Yong ; Brian K. Pikul ; Laura Marcu, et al.
"Intraoperative delineation of primary brain tumors using time-resolved fluorescence spectroscopy", J. Biomed. Opt. 15(2), 027008 (April 14, 2010). ; http://dx.doi.org/10.1117/1.3374049


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