Research Papers: Imaging

Ex vivo imaging and quantification of liver fibrosis using second-harmonic generation microscopy

[+] Author Affiliations
Tzu-Lin Sun, Yuan Liu, Ming-Chin Sung

National Taiwan University, Department of Physics, Taipei, 10617 Taiwan

Hsiao-Ching Chen

National Taiwan University Hospital, Department of Internal Medicine, Taipei, 100 Taiwan and National Taiwan University, College of Medicine, Taipei, 100 Taiwan

Chun-Hui Yang, Vladimir Hovhannisyan

National Taiwan University, Department of Physics, Taipei, 10617 Taiwan

Wei-Chou Lin, Yung-Ming Jeng

National Taiwan University Hospital and National Taiwan University, College of Medicine, Department of Pathology, Taipei, 10002 Taiwan

Wei-Liang Chen

National Taiwan University, Department of Physics, Taipei, 10617 Taiwan

Ling-Ling Chiou, Guan-Tarn Huang

National Taiwan University Hospital, Department of Internal Medicine, Taipei, 100 Taiwan and National Taiwan University, College of Medicine, Taipei, 100 Taiwan

Ki-Hean Kim, Peter T. C. So

Massachusetts Institute of Technology, Department of Mechanical Engineering and Division of Bioengineering, Cambridge, Massachusetts 02139

Yang-Fang Chen

National Taiwan University, Department of Physics, Taipei, 10617 Taiwan

Hsuan-Shu Lee

National Taiwan University, Institute of Biotechnology and National Taiwan University Hospital, Department of Internal Medicine and National Taiwan University, College of Medicine, Taipei, 100 Taiwan

Chen-Yuan Dong

National Taiwan University, Department of Physics and , Center for Quantum Science and Engineering and , Division of Genomic Medicine, Research Center for Medical Excellence, Biomedical Molecular Imaging Core, Taipei, 100 Taiwan

J. Biomed. Opt. 15(3), 036002 (June 30, 2010). doi:10.1117/1.3427146
History: Received November 12, 2009; Revised February 25, 2010; Accepted March 09, 2010; Published June 30, 2010; Online June 30, 2010
Text Size: A A A

Conventionally, liver fibrosis is diagnosed using histopathological techniques. The traditional method is time-consuming in that the specimen preparation procedure requires sample fixation, slicing, and labeling. Our goal is to apply multiphoton microscopy to efficiently image and quantitatively analyze liver fibrosis specimens bypassing steps required in histological preparation. In this work, the combined imaging modality of multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) was used for the qualitative imaging of liver fibrosis of different METAVIR grades under label-free, ex vivo conditions. We found that while MAF is effective in identifying cellular architecture in the liver specimens, it is the spectrally distinct SHG signal that allows the characterization of the extent of fibrosis. We found that qualitative SHG imaging can be used for the effective identification of the associated features of liver fibrosis specimens graded METAVIR 0 to 4. In addition, we attempted to associate quantitative SHG signal to the different METAVIR grades and found that an objective determination of the extent of disease progression can be made. Our approach demonstrates the potential of using multiphoton imaging in rapid classification of ex vivo liver fibrosis in the clinical setting and investigation of liver fibrosis–associated physiopathology in animal models in vivo.

Figures in this Article
E800, Nikon, Tokyo, Japan

Citation

Tzu-Lin Sun ; Yuan Liu ; Ming-Chin Sung ; Hsiao-Ching Chen ; Chun-Hui Yang, et al.
"Ex vivo imaging and quantification of liver fibrosis using second-harmonic generation microscopy", J. Biomed. Opt. 15(3), 036002 (June 30, 2010). ; http://dx.doi.org/10.1117/1.3427146


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.