Research Papers: Imaging

Lookup-table method for imaging optical properties with structured illumination beyond the diffusion theory regime

[+] Author Affiliations
Tim A. Erickson

University of Texas, Department of Biomedical Engineering, 107 W Dean Keeton, Austin, Texas 78712

Amaan Mazhar

Beckman Laser Institute, 1002 Health Sciences Road, Irvine, California 92617-3010

David Cuccia

Modulated Imaging, Inc., 1002 Health Sciences Road, Irvine, California 92617-3010

Anthony J. Durkin

Beckman Laser Institute, 1002 Health Sciences Road, Irvine, California 92617-3010

James W. Tunnell

University of Texas, Department of Biomedical Engineering, 107 W Dean Keeton, Austin, Texas 78712

J. Biomed. Opt. 15(3), 036013 (May 24, 2010). doi:10.1117/1.3431728
History: Received January 26, 2010; Revised March 25, 2010; Accepted April 05, 2010; Published May 24, 2010; Online May 24, 2010
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Sinusoidally structured illumination is used in concert with a phantom-based lookup-table (LUT) to map wide-field optical properties in turbid media with reduced albedos as low as 0.44. A key advantage of the lookup-table approach is the ability to measure the absorption (μa) and reduced scattering coefficients (μs) over a much broader range of values than permitted by current diffusion theory methods. Through calibration with a single reflectance standard, the LUT can extract μs from 0.8to2.4mm1 with an average root-mean-square (rms) error of 7% and extract μa from 0to1.0mm1 with an average rms error of 6%. The LUT is based solely on measurements of two parameters, reflectance R and modulation M at an illumination period of 10mm. A single set of three phase-shifted images is sufficient to measure both M and R, which are then used to generate maps of absorption and scattering by referencing the LUT. We establish empirically that each pair (M,R) maps uniquely to only one pair of (μs,μa) and report that the phase function (i.e., size) of the scatterers can influence the accuracy of optical property extraction.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Tim A. Erickson ; Amaan Mazhar ; David Cuccia ; Anthony J. Durkin and James W. Tunnell
"Lookup-table method for imaging optical properties with structured illumination beyond the diffusion theory regime", J. Biomed. Opt. 15(3), 036013 (May 24, 2010). ; http://dx.doi.org/10.1117/1.3431728


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