Research Papers: Imaging

Volumetric in vivo imaging of intracochlear microstructures in mice by high-speed spectral domain optical coherence tomography

[+] Author Affiliations
Hrebesh M. Subhash, Viviana Davila

Oregon Health and Science University, School of Medicine, Department of Biomedical Engineering, Biophotonics and Imaging Laboratory, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239

Hai Sun

Oregon Health and Science University, School of Medicine, Department of Biomedical Engineering, Biophotonics and Imaging Laboratory and Department of Neurological Surgery, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239

Anh T. Nguyen-Huynh

Oregon Health and Science University, School of Medicine, Department of Otolaryngology-Head and Neck Surgery, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239

Alfred L. Nuttall

Oregon Health and Science University, School of Medicine, Oregon Hearing Research Center, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239

Ruikang K. Wang

Oregon Health and Science University, School of Medicine, Department of Biomedical Engineering, Biophotonics and Imaging Laboratory and Department of Anesthesiology & Peri-operative Medicine, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239

J. Biomed. Opt. 15(3), 036024 (June 30, 2010). doi:10.1117/1.3456554
History: Received January 14, 2010; Revised April 29, 2010; Accepted May 06, 2010; Published June 30, 2010; Online June 30, 2010
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There is considerable interest in developing new methods for in vivo imaging of the complex anatomy of the mammalian cochlea for clinical as well as fundamental studies. In this study, we explored, the feasibility of spectral domain optical coherence tomography (SD-OCT) for 3-D in vivo imaging of the cochlea in mice. The SD-OCT system employed in this study used a broadband light source centered at 1300nm, and the imaging speed of the system was 47,000 A-scans per second using the InGaAs camera. The system was capable of providing fully processed, high-resolution B-scan images [512 (axial)×128 (lateral) pixels] at 280 frames per sec. The 3-D imaging acquisition time for a whole cochlea was 0.45sec. The traditional SD-OCT structural imaging algorithm was used to reconstruct 3-D cochlear morphology. We demonstrated that SD-OCT can be successfully used for in vivo imaging of important morphological features within the mouse cochlea, such as the otic capsule and structures within, including Reissner’s membrane, the basilar membrane, tectorial membrane, organ of Corti, and modiolus of the apical and middle turns.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Hrebesh M. Subhash ; Viviana Davila ; Hai Sun ; Anh T. Nguyen-Huynh ; Alfred L. Nuttall, et al.
"Volumetric in vivo imaging of intracochlear microstructures in mice by high-speed spectral domain optical coherence tomography", J. Biomed. Opt. 15(3), 036024 (June 30, 2010). ; http://dx.doi.org/10.1117/1.3456554


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