Research Papers: Sensing

Biochemical analysis and quantification of hematopoietic stem cells by infrared spectroscopy

[+] Author Affiliations
Udi Zelig

Ben-Gurion University of the Negev, Department of Biomedical Engineering, Beer-Sheva 84105 Israel

Ziv Dror

Soroka University Medical Center, Department of Pediatric Hemato-Oncology, Beer-Sheva 84101 Israel

Svetlana Iskovich

Schneider Children’s Medical Center, Department of Pediatric Hematology-Oncology, Center for Stem Cell Research, Petach Tikva 49202 Israel

Amir Zwielly

Ben-Gurion University of the Negev, Department of Physics, Beer-Sheva 84105 Israel

Miri Ben-Harush

Soroka University Medical Center, Department of Pediatric Hemato-Oncology, Beer-Sheva 84101 Israel

Ilana Nathan

Ben-Gurion University of the Negev, Department of Clinical Biochemistry, Beer-Sheva 84105 Israel and Soroka University Medical Center, Institute of Hematology, Beer-Sheva 84101 Israel

Shaul Mordechai

Ben-Gurion University of the Negev, Department of Physics and Cancer Research Center, Beer-Sheva 84105 Israel

Joseph Kapelushnik

Soroka University Medical Center, Department of Pediatric Hemato-Oncology, Beer-Sheva 84101 Israel

J. Biomed. Opt. 15(3), 037008 (June 09, 2010). doi:10.1117/1.3442728
History: Received November 04, 2009; Revised March 26, 2010; Accepted April 07, 2010; Published June 09, 2010; Online June 09, 2010
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Identification of hematopoietic stem cells (HSCs) in different stages of maturation is one of the major issues in stem cell research and bone marrow (BM) transplantation. Each stage of maturation of HSCs is characterized by a series of distinct glycoproteins present on the cell plasma membrane surface, named a cluster of differentiation (CD). Currently, complicated and expensive procedures based on CD expression are needed for identification and isolation of HSCs. This method is under dispute, since the correct markers’ composition is not strictly clear, thus there is need for a better method for stem cell characterization. In the present study, Fourier transform infrared (FTIR) spectroscopy is employed as a novel optical method for identification and characterization of HSCs based on their entire biochemical features. FTIR spectral analysis of isolated mice HSCs reveals several spectral markers related to lipids, nucleic acids, and carbohydrates, which distinguish HSCs from BM cells. The unique “open” conformation of HSC DNA as identified by FTIR is exploited for HSCs quantification in the BM. The proposed method of FTIR spectroscopy for HSC identification and quantification can contribute to stem cell research and BM transplantation.

Figures in this Article
© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Udi Zelig ; Ziv Dror ; Svetlana Iskovich ; Amir Zwielly ; Miri Ben-Harush, et al.
"Biochemical analysis and quantification of hematopoietic stem cells by infrared spectroscopy", J. Biomed. Opt. 15(3), 037008 (June 09, 2010). ; http://dx.doi.org/10.1117/1.3442728


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