Special Section on Selected Topics in Biophotonics: Photodynamic Therapy and Optical Micromanipulation for Biophotonics

In vivo carotid artery closure by laser activation of hyaluronan-embedded gold nanorods

[+] Author Affiliations
Paolo Matteini, Fulvio Ratto, Francesca Rossi

Institute of Applied Physics “Nello Carrara”, Italian National Research Council, via Madonna del Piano, 10, 50019 Sesto Fiorentino, Italy

Giacomo Rossi

University of Camerino, Faculty of Veterinary Medicine, via Circonvallazione, 93/95, 62024 Matelica, Italy

Giuseppe Esposito, Alfredo Puca, Alessio Albanese, Giulio Maira

Catholic University School of Medicine, Institute of Neurosurgery, L.go A. Gemelli, 8, 00168 Rome, Italy

Roberto Pini

Institute of Applied Physics “Nello Carrara”, Italian National Research Council, via Madonna del Piano, 10, 50019 Sesto Fiorentino, Italy

J. Biomed. Opt. 15(4), 041508 (July 06, 2010). doi:10.1117/1.3449574
History: Received October 10, 2009; Revised March 01, 2010; Accepted April 09, 2010; Published July 06, 2010; Online July 06, 2010
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We prove the first application of near-infrared-absorbing gold nanorods (GNRs) for in vivo laser closure of a rabbit carotid artery. GNRs are first functionalized with a biopolymeric shell and then embedded in hyaluronan, which gives a stabilized and handy laser-activable formulation. Four rabbits undergo closure of a 3-mm longitudinal incision performed on the carotid artery by means of a 810-nm diode laser in conjunction with the topical application of the GNRs composite. An effective surgery is obtained by using a 40-Wcm2 laser power density. The histological and electron microscopy evaluation after a 30-day follow-up demonstrates complete healing of the treated arteries with full re-endothelization at the site of GNRs application. The absence of microgranuloma formation and/or dystrophic calcification is evidence that no host reaction to nanoparticles interspersed through the vascular tissue occurred. The observation of a reshaping and associated blue shift of the NIR absorption band of GNRs after laser treatment supports the occurrence of a self-terminating process, and thus of additional safety of the minimally invasive laser procedure. This study underlines the feasibility of using GNRs for in vivo laser soldering applications, which represents a step forward toward the introduction of nanotechnology-based therapies in minimally invasive clinical practices.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Paolo Matteini ; Fulvio Ratto ; Francesca Rossi ; Giacomo Rossi ; Giuseppe Esposito, et al.
"In vivo carotid artery closure by laser activation of hyaluronan-embedded gold nanorods", J. Biomed. Opt. 15(4), 041508 (July 06, 2010). ; http://dx.doi.org/10.1117/1.3449574


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