Research Papers: General

Real-time observation of the effect of iron on receptor-mediated endocytosis of transferrin conjugated with quantum dots

[+] Author Affiliations
Hai-Li Zhang, Yong-Qiang Li, Ming-Zhen Zhang, Yuan-Di Zhao

Huazhong University of Science and Technology, Wuhan National Laboratory for Optoelectronics, Britton Chance Center for Biomedical Photonics, Wuhan, Hubei 430074, China

J. Biomed. Opt. 15(4), 045003 (August 11, 2010). doi:10.1117/1.3465595
History: Received January 27, 2010; Revised May 06, 2010; Accepted May 19, 2010; Published August 11, 2010; Online August 11, 2010
Text Size: A A A

The optical properties of antiphotobleaching and the advantage of long-term fluorescence observation of quantum dots are fully adopted to study the effects of iron on the endocytosis of transferrin. Quantum dots are labeled for transferrin and endocytosis of transferrin in HeLa cells is observed under the normal state, iron overloading, and an iron-deficient state. In these three states, the fluorescence undergoes a gradual process of first dark, then light, and finally dark, indicating the endocytosis of transferrin. The fluorescence intensity analysis shows that a platform emerges when fluorescence changes to a certain degree in the three states. Experienced a same period of time after platform, the fluorescence strength of cells in the normal state is 1.2 times the first value, and the iron-deficiency state is 1.4 times, but the iron overloading state was 0.85 times. We also find that the average fluorescence intensity in cells detected by the spectrophotometer in the iron-deficiency state is almost 7 times than that in a high iron state. All this proves that iron overloading would slow the process, but iron deficiency would accelerate endocytosis. We advance a direct observational method that may contribute to further study of the relationship of iron and transferrin.

Figures in this Article
© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Hai-Li Zhang ; Yong-Qiang Li ; Ming-Zhen Zhang and Yuan-Di Zhao
"Real-time observation of the effect of iron on receptor-mediated endocytosis of transferrin conjugated with quantum dots", J. Biomed. Opt. 15(4), 045003 (August 11, 2010). ; http://dx.doi.org/10.1117/1.3465595


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.