Research Papers: General

Silver nanoparticle-induced degranulation observed with quantitative phase microscopy

[+] Author Affiliations
Wenzhong Yang, Seungrag Lee, Jiyong Lee, Yoonsung Bae, Dugyoung Kim

Department of Information and Communications, Gwangju Institute of Science and Technology, 1 Oryong-dong, Buk-gu, Gwangju 500-712, Republic of Korea

J. Biomed. Opt. 15(4), 045005 (July 29, 2010). doi:10.1117/1.3470104
History: Received November 10, 2009; Revised June 01, 2010; Accepted June 03, 2010; Published July 29, 2010; Online July 29, 2010
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Monitoring a degranulation process in a live mast cell is a quite important issue in immunology and pharmacology. Because the size of a granule is normally much smaller than the resolution limit of an optical microscope system, there is no direct real-time live cell imaging technique for observing degranulation processes except for fluorescence imaging techniques. In this research, we propose optical quantitative phase microscopy (QPM) as a new observation tool to study degranulation processes in a live mast cell without any fluorescence labeling. We measure the cell volumes and the cross sectional profiles (x-z plane) of an RBL-2H3 cell and a HeLa cell, before and after they are exposed to calcium ionophore A23187 and silver nanoparticles (AgNPs). We verify that the volume and the cross sectional line profile of the RBL-2H3 cell were changed significantly when it was exposed to A23187. When 50μgmL of AgNP is used instead of A23187, the measurements of cell volume and cross sectional profiles indicate that RBL-2H3 cells also follow degranulation processes. Degranulation processes for these cells are verified by monitoring the increase of intracellular calcium ([Ca2+]i) and histamine with fluorescent methods.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Wenzhong Yang ; Seungrag Lee ; Jiyong Lee ; Yoonsung Bae and Dugyoung Kim
"Silver nanoparticle-induced degranulation observed with quantitative phase microscopy", J. Biomed. Opt. 15(4), 045005 (July 29, 2010). ; http://dx.doi.org/10.1117/1.3470104


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