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Research Papers: Imaging

Virtual biopsy of rat tympanic membrane using higher harmonic generation microscopy

[+] Author Affiliations
Wen-Jeng Lee

National Taiwan University, Department of Electrical Engineering, Taipei, 10617, Taiwan and National Taiwan University Hospital, Department of Medical Imaging, Taipei, 10051, Taiwan

Chia-Fone Lee

Tzu Chi General Hospital, Department of Otolaryngology, Hualien, 97004, Taiwan and Tzu Chi University, Department of Medicine, Hualien, 97004, Taiwan

Szu-Yu Chen

National Taiwan University, Department of Electrical Engineering, Taipei, 10617, Taiwan and National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Taipei, 10617, Taiwan

Yuh-Shyang Chen

Tai-Tung Christian Hospital, Department of Otolaryngology, Tai-Tung, 95048, Taiwan

Chi-Kuang Sun

National Taiwan University, Department of Electrical Engineering, Taipei, 10617, Taiwan and National Taiwan University, Graduate Institute of Photonics and Optoelectronics, Taipei, 10617, Taiwan and Academia Sinica, Research Center for Applied Sciences, Taipei 11529, Taiwan

J. Biomed. Opt. 15(4), 046012 (August 19, 2009May 29, 2010June 02, 2010August 02, 2010August 02, 2010). doi:10.1117/1.3469848
History: Received August 19, 2009; Revised May 29, 2010; Accepted June 02, 2010; Published August 02, 2010; Online August 02, 2010
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Multiharmonic optical microscopy has been widely applied in biomedical research due to its unique capability to perform noninvasive studies of biomaterials. In this study, virtual biopsy based on back-propagating multiple optical harmonics, combining second and third harmonics, is applied in unfixed rat tympanic membrane. We show that third harmonic generation can provide morphologic information on the epithelial layers of rat tympanic membrane as well as radial collagen fibers in middle fibrous layers, and that second harmonic generation can provide information on both radial and circular collagen fibers in middle fibrous layers. Through third harmonic generation, the capillary and red blood cells in the middle fibrous layer are also noted. Additionally, the 3-D relationship to adjacent bony structures and spatial variations in thickness and curvature are obtained. Our study demonstrates the feasibility of using a noninvasive optical imaging system for comprehensive evaluation of the tympanic membrane.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Wen-Jeng Lee ; Chia-Fone Lee ; Szu-Yu Chen ; Yuh-Shyang Chen and Chi-Kuang Sun
"Virtual biopsy of rat tympanic membrane using higher harmonic generation microscopy", J. Biomed. Opt. 15(4), 046012 (August 19, 2009May 29, 2010June 02, 2010August 02, 2010August 02, 2010). ; http://dx.doi.org/10.1117/1.3469848


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