Research Papers: Imaging

Shack-Hartmann wavefront-sensor-based adaptive optics system for multiphoton microscopy

[+] Author Affiliations
Jae Won Cha

Massachusetts Institute of Technology, Department of Mechanical Engineering, 500 Technology Square, NE47-276, Cambridge, Massachusetts 02139

Jerome Ballesta

Imagine Optic, 18 rue Charles de Gaulle, Orsay, France 91400

Peter T. C. So

Massachusetts Institute of Technology, Department of Mechanical Engineering and Biological Engineering, 500 Technology Square, NE47-279, Cambridge, Massachusetts 02139

J. Biomed. Opt. 15(4), 046022 (August 20, 2010). doi:10.1117/1.3475954
History: Received February 03, 2010; Revised June 12, 2010; Accepted June 23, 2010; Published August 20, 2010; Online August 20, 2010
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The imaging depth of two-photon excitation fluorescence microscopy is partly limited by the inhomogeneity of the refractive index in biological specimens. This inhomogeneity results in a distortion of the wavefront of the excitation light. This wavefront distortion results in image resolution degradation and lower signal level. Using an adaptive optics system consisting of a Shack-Hartmann wavefront sensor and a deformable mirror, wavefront distortion can be measured and corrected. With adaptive optics compensation, we demonstrate that the resolution and signal level can be better preserved at greater imaging depth in a variety of ex-vivo tissue specimens including mouse tongue muscle, heart muscle, and brain. However, for these highly scattering tissues, we find signal degradation due to scattering to be a more dominant factor than aberration.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Jae Won Cha ; Jerome Ballesta and Peter T. C. So
"Shack-Hartmann wavefront-sensor-based adaptive optics system for multiphoton microscopy", J. Biomed. Opt. 15(4), 046022 (August 20, 2010). ; http://dx.doi.org/10.1117/1.3475954


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