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Research Papers: Imaging

Superresolved digital in-line holographic microscopy for high-resolution lensless biological imaging

[+] Author Affiliations
Vicente Micó

Universitat de Valencia, Departamento de Óptica, C/Dr. Moliner 50, 46100 Burjassot, Spain

Zeev Zalevsky

Bar-Ilan University, School of Engineering, Ramat-Gan 52900, Israel

J. Biomed. Opt. 15(4), 046027 (August 19, 2010). doi:10.1117/1.3481142
History: Received February 13, 2010; Revised June 28, 2010; Accepted July 01, 2010; Published August 19, 2010; Online August 19, 2010
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Digital in-line holographic microscopy (DIHM) is a modern approach capable of achieving micron-range lateral and depth resolutions in three-dimensional imaging. DIHM in combination with numerical imaging reconstruction uses an extremely simplified setup while retaining the advantages provided by holography with enhanced capabilities derived from algorithmic digital processing. We introduce superresolved DIHM incoming from time and angular multiplexing of the sample spatial frequency information and yielding in the generation of a synthetic aperture (SA). The SA expands the cutoff frequency of the imaging system, allowing submicron resolutions in both transversal and axial directions. The proposed approach can be applied when imaging essentially transparent (low-concentration dilutions) and static (slow dynamics) samples. Validation of the method for both a synthetic object (U.S. Air Force resolution test) to quantify the resolution improvement and a biological specimen (sperm cells biosample) are reported showing the generation of high synthetic numerical aperture values working without lenses.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Vicente Micó and Zeev Zalevsky
"Superresolved digital in-line holographic microscopy for high-resolution lensless biological imaging", J. Biomed. Opt. 15(4), 046027 (August 19, 2010). ; http://dx.doi.org/10.1117/1.3481142


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