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Research Papers: Sensing

Changes of collagen and nicotinamide adenine dinucleotide in human cancerous and normal prostate tissues studied using native fluorescence spectroscopy with selective excitation wavelength

[+] Author Affiliations
Yang Pu

City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Departments of Electrical Engineering, 160 Convent Avenue, New York, New York 10031

Wubao Wang, Guichen Tang, Robert R. Alfano

City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Department of Physics, 160 Convent Avenue, New York, New York 10031

J. Biomed. Opt. 15(4), 047008 (July 23, 2010). doi:10.1117/1.3463479
History: Received March 05, 2010; Revised May 19, 2010; Accepted May 21, 2010; Published July 23, 2010; July 30, 2010; Online September 08, 2010
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The fluorescence spectra of human cancerous and normal prostate tissues obtained by the selective excitation wavelength of 340nm were measured. The contributions of principle biochemical components to tissue fluorescence spectra were investigated using the method of multivariate curve resolution with alternating least squares. The results show that there is a reduced contribution from the emission of collagen and increased contribution from nicotinamide adenine dinucleotide (NADH) in cancerous tissues as compared with normal tissue. This difference is attributed to the changes of relative contents of NADH and collagen during cancer development. This research may present a potential native biomarker for prostate cancer detection.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Yang Pu ; Wubao Wang ; Guichen Tang and Robert R. Alfano
"Changes of collagen and nicotinamide adenine dinucleotide in human cancerous and normal prostate tissues studied using native fluorescence spectroscopy with selective excitation wavelength", J. Biomed. Opt. 15(4), 047008 (July 23, 2010). ; http://dx.doi.org/10.1117/1.3463479


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