Research Papers: Imaging

Imaging of normal and pathologic joint synovium using nonlinear optical microscopy as a potential diagnostic tool

[+] Author Affiliations
Nivedan Tiwari

University of California, Irvine, Beckman Laser Institute, 1002 Health Sciences Road, Irvine, California 92612

Sanjay Chabra

3943 Irvine Boulevard #335, Irvine, California 92602

Sheherbano Mehdi

23441 Madison Avenue, Suite 340, Torrance, California 90505

Paula Sweet, Tatiana B. Krasieva

University of California, Irvine, Beckman Laser Institute, 1002 Health Sciences Road, Irvine, California 92612

Roy Pool

Texas A&M University, College of Veterinary Medicine, Department of Veterinary Pathobiology, College Station, Texas 77843-4467

Brian Andrews

University of Notre Dame Australia, School of Medicine, Fremantle, 47 Henry Street (PO Box 1225), Fremantle, Western Australia 6959

George M. Peavy

University of California, Irvine, Beckman Laser Institute, 1002 Health Sciences Road, Irvine, California 92612

J. Biomed. Opt. 15(5), 056001 (September 13, 2010). doi:10.1117/1.3484262
History: Received March 17, 2010; Revised July 06, 2010; Accepted July 14, 2010; Published September 13, 2010; Online September 13, 2010
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An estimated 1.3 million people in the United States suffer from rheumatoid arthritis (RA). RA causes profound changes in the synovial membrane of joints, and without early diagnosis and intervention, progresses to permanent alterations in joint structure and function. The purpose of this study is to determine if nonlinear optical microscopy (NLOM) can utilize the natural intrinsic fluorescence properties of tissue to generate images that would allow visualization of the structural and cellular composition of fresh, unfixed normal and pathologic synovial tissue. NLOM is performed on rabbit knee joint synovial samples using 730- and 800-nm excitation wavelengths. Less than 30mW of excitation power delivered with a 40×, 0.8-NA water immersion objective is sufficient for the visualization of synovial structures to a maximum depth of 70μm without tissue damage. NLOM imaging of normal and pathologic synovial tissue reveals the cellular structure, synoviocytes, adipocytes, collagen, vascular structures, and differential characteristics of inflammatory infiltrates without requiring tissue processing or staining. Further study to evaluate the ability of NLOM to assess the characteristics of pathologic synovial tissue and its potential role for the management of disease is warranted.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Nivedan Tiwari ; Sanjay Chabra ; Sheherbano Mehdi ; Paula Sweet ; Tatiana B. Krasieva, et al.
"Imaging of normal and pathologic joint synovium using nonlinear optical microscopy as a potential diagnostic tool", J. Biomed. Opt. 15(5), 056001 (September 13, 2010). ; http://dx.doi.org/10.1117/1.3484262


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