Research Papers: Imaging

Human graft cornea and laser incisions imaging with micrometer scale resolution full-field optical coherence tomography

[+] Author Affiliations
Gaël Latour, Gaëlle Georges, Laure Siozade Lamoine, Carole Deumié

Aix-Marseille Université, Ecole Centrale Marseille, CNRS, Institut Fresnel, Domaine Universitaire de Saint-Jérôme, 13397 Marseille Cedex 20, France

John Conrath, Louis Hoffart

Aix-Marseille Université, Hôpital de la Timone, Service d’Ophtalmologie, 264 rue Saint Pierre, 13385 Marseille Cedex 5, France

J. Biomed. Opt. 15(5), 056006 (September 17, 2010). doi:10.1117/1.3486544
History: Received March 04, 2010; Revised June 25, 2010; Accepted July 26, 2010; Published September 17, 2010; Online September 17, 2010
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Micrometer scale resolution full-field optical coherence tomography (FF-OCT) is developed for imaging human graft corneas. Three-dimensional (3-D) images with ultrahigh resolution (respectively, 1 and 1.5μm in the axial and transverse directions), comparable to traditional histological sections, are obtained allowing the visualization of the cells and the precise structure of the different layers that compose the tissue. The sensitivity of our device enables imaging the entire thickness of the cornea, even in edematous corneas more than 800μm thick. Furthermore, we provide tomographic 3-D images of laser incisions inside the tissue at various depths without slicing the studied corneas. The effects of laser ablations can be observed, along various optical sections, directly in the bulk of the sample with high accuracy, providing information on the interface quality and also imaging tiny changes of the tissue structure. FF-OCT appears to be a powerful tool for subcellular imaging of the corneal structure and pathologies on the entire thickness of the tissue as well as interface quality and changes in the collagen structure due to laser incisions on ex vivo human cornea.

Figures in this Article
© 2010 Society of Photo-Optical Instrumentation Engineers

Topics

Cornea ; Lasers ; Tissues

Citation

Gaël Latour ; Gaëlle Georges ; Laure Siozade Lamoine ; Carole Deumié ; John Conrath, et al.
"Human graft cornea and laser incisions imaging with micrometer scale resolution full-field optical coherence tomography", J. Biomed. Opt. 15(5), 056006 (September 17, 2010). ; http://dx.doi.org/10.1117/1.3486544


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