Research Papers: Imaging

Nonnegative matrix factorization: a blind spectra separation method for in vivo fluorescent optical imaging

[+] Author Affiliations
Anne-Sophie Montcuquet

CEA-LETI, Minatec, 17 rue des Martyrs, Grenoble Cedex 9, 38054, France and GIPSA-Lab/DIS, CNRS, UMR 5216, BP Saint Martin d’Hères Cedex, France

Lionel Hervé, Fabrice Navarro, Jean-Marc Dinten

CEA-LETI, Minatec, 17 rue des Martyrs, Grenoble Cedex 9, 38054, France

Jérôme I. Mars

GIPSA-Lab/DIS, CNRS, UMR 5216, BP Saint Martin d’Hères Cedex, France

J. Biomed. Opt. 15(5), 056009 (September 30, 2010). doi:10.1117/1.3491796
History: Received January 27, 2010; Revised July 21, 2010; Accepted August 16, 2010; Published September 30, 2010; Online September 30, 2010
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Fluorescence imaging in diffusive media is an emerging imaging modality for medical applications that uses injected fluorescent markers that bind to specific targets, e.g., carcinoma. The region of interest is illuminated with near-IR light and the emitted back fluorescence is analyzed to localize the fluorescence sources. To investigate a thick medium, as the fluorescence signal decreases with the light travel distance, any disturbing signal, such as biological tissues intrinsic fluorescence (called autofluorescence) is a limiting factor. Several specific markers may also be simultaneously injected to bind to different molecules, and one may want to isolate each specific fluorescent signal from the others. To remove the unwanted fluorescence contributions or separate different specific markers, a spectroscopic approach is explored. The nonnegative matrix factorization (NMF) is the blind positive source separation method we chose. We run an original regularized NMF algorithm we developed on experimental data, and successfully obtain separated in vivo fluorescence spectra.

© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Anne-Sophie Montcuquet ; Lionel Hervé ; Fabrice Navarro ; Jean-Marc Dinten and Jérôme I. Mars
"Nonnegative matrix factorization: a blind spectra separation method for in vivo fluorescent optical imaging", J. Biomed. Opt. 15(5), 056009 (September 30, 2010). ; http://dx.doi.org/10.1117/1.3491796


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