Research Papers: Imaging

Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery

[+] Author Affiliations
Yinghua Sun

University of California, Davis, Department of Biomedical Engineering, Davis, California 95616 and National Science Foundation, Center for Biophotonics Science & Technology, Sacramento, California 95817

Nisa Hatami, Matthew Yee, Jennifer Phipps

University of California, Davis, Department of Biomedical Engineering, Davis, California 95616

Daniel S. Elson

Imperial College London, Institute of Biomedical Engineering, London SW7 2AZ, United Kingdom

Fredric Gorin

University of California, Davis, Department of Neurology, Davis, California 95616

Rudolph J. Schrot

University of California, Davis, Department of Neurological Surgery, Sacramento, California 95817

Laura Marcu

University of California, Davis, Department of Biomedical Engineering, Davis, California 95616 and National Science Foundation, Center for Biophotonics Science & Technology, Sacramento, California 95817

J. Biomed. Opt. 15(5), 056022 (October 07, 2010). doi:10.1117/1.3486612
History: Received June 07, 2010; Revised August 10, 2010; Accepted August 12, 2010; Published October 07, 2010; Online October 07, 2010
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We demonstrate for the first time the application of an endoscopic fluorescence lifetime imaging microscopy (FLIM) system to the intraoperative diagnosis of glioblastoma multiforme (GBM). The clinically compatible FLIM prototype integrates a gated (down to 0.2ns) intensifier imaging system with a fiber-bundle (fiber image guide of 0.5mm diameter, 10,000 fibers with a gradient index lens objective 0.5 NA, and 4mm field of view) to provide intraoperative access to the surgical field. Experiments conducted in three patients undergoing craniotomy for tumor resection demonstrate that FLIM-derived parameters allow for delineation of tumor from normal cortex. For example, at 460±25-nm wavelength band emission corresponding to NADH/NADPH fluorescence, GBM exhibited a weaker florescence intensity (35% less, p-value <0.05) and a longer lifetime τGBM-Amean=1.59±0.24ns than normal cortex τNC-Amean=1.28±0.04ns (p-value <0.005). Current results demonstrate the potential use of FLIM as a tool for image-guided surgery of brain tumors.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Yinghua Sun ; Nisa Hatami ; Matthew Yee ; Jennifer Phipps ; Daniel S. Elson, et al.
"Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery", J. Biomed. Opt. 15(5), 056022 (October 07, 2010). ; http://dx.doi.org/10.1117/1.3486612


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