Special Section on Optical Diagnostic and Biophotonic Methods from Bench to Bedside

Diffuse reflectance spectroscopy for in vivo pediatric brain tumor detection

[+] Author Affiliations
Wei-Chiang Lin

Miami Children's Hospitaland and Florida International University, Department of Biomedical Engineering, 10555 West Flagler Street, EAS 2673, Miami, Florida 33131

David I. Sandberg, Sanjiv Bhatia

Miami Children's Hospitaland and University of Miami Leonard M. Miller School of Medicine, Department of Neurological Surgery, 3215 SW 62nd Avenue, Ambulatory Care Building, Suite 3109, Miami, Florida 33155

Mahlon Johnson

University of Rochester Medical Center, Department of Pathology, Division of Neuropathology, Box 626, 601 Elmwood Avenue, Rochester, New York 14642

Sanghoon Oh

Miami Children's Hospitaland and Florida International University, Department of Biomedical Engineering, 10555 West Flagler Street, EAS 2673, Miami, Florida 33131

John Ragheb

Miami Children's Hospitaland and University of Miami Leonard M. Miller School of Medicine, Department of Neurological Surgery, 3215 SW 62nd Avenue, Ambulatory Care Building, Suite 3109, Miami, Florida 33155

J. Biomed. Opt. 15(6), 061709 (November 19, 2010). doi:10.1117/1.3505012
History: Received February 01, 2010; Revised September 03, 2010; Accepted September 10, 2010; Published November 19, 2010; Online November 19, 2010
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The concept of using diffuse reflectance spectroscopy to distinguish intraoperatively between pediatric brain tumors and normal brain parenchyma at the edge of resection cavities is evaluated using an in vivo human study. Diffuse reflectance spectra are acquired from normal and tumorous brain areas of 12 pediatric patients during their tumor resection procedures, using a spectroscopic system with a handheld optical probe. A total of 400 spectra are acquired at the rate of 33 Hz from a single investigated site, from which the mean spectrum and the standard deviation are calculated. The mean diffuse reflectance spectra collected are divided into the normal and the tumorous categories in accordance with their corresponding results of histological analysis. Statistical methods are used to identify those spectral features that effectively separated the two tissue categories, and to quantify the spectral variations induced by the motion of the handheld probe during a single spectral acquisition procedure. The results show that diffuse reflectance spectral intensities between 600 and 800 nm are effective in terms of differentiating normal cortex from brain tumors. Furthermore, probe movements induce large variations in spectral intensities (i.e., larger standard deviation) between 400 and 600 nm.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Wei-Chiang Lin ; David I. Sandberg ; Sanjiv Bhatia ; Mahlon Johnson ; Sanghoon Oh, et al.
"Diffuse reflectance spectroscopy for in vivo pediatric brain tumor detection", J. Biomed. Opt. 15(6), 061709 (November 19, 2010). ; http://dx.doi.org/10.1117/1.3505012


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