Special Section on Optical Diagnostic and Biophotonic Methods from Bench to Bedside

Simple and objective method for routine detection of the macular pigment xanthophyll

[+] Author Affiliations
Dietrich Schweitzer, Susanne Jentsch, Jens Dawczynski, Martin Hammer

Augenklinik der Friedrich-Schiller-Universität Jena, Experimentelle Ophthalmologie Bachstrasse 18 07743 Jena, Germany

Ute E.K. Wolf-Schnurrbusch, Sebastian Wolf

University of Bern, University of eye clinic Inselspital Klinik für Augenheilkunde Bern, Switzerland 3010

J. Biomed. Opt. 15(6), 061714 (December 17, 2010). doi:10.1117/1.3526358
History: Received February 15, 2010; Revised October 25, 2010; Accepted November 01, 2010; Published December 17, 2010; Online December 17, 2010
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A new simple method for two-dimensional determination of optical density of macular pigment xanthophyll (ODx) in clinical routine is based on a single blue-reflection fundus image. Individual different vignetting is corrected by a shading function. For its construction, nodes are automatically found in structureless image regions. The influence of stray light in elderly crystalline lenses is compensated by a correction function that depends on age. The reproducibility of parameters in a one-wavelength reflection method determined for three subjects (47, 61, and 78 years old) was: maxODx = 6.3%, meanODx = 4.6%, volume = 6%, and area = 6% already before stray-light correction. ODx was comparable in pseudophakic and in an eye with a crystalline lens of the same 11 subjects after stray-light correction. Significant correlation in ODx was found between the one-wavelength reflection method and the two-wavelength autofluorescence method for pseudophakic and cataract eyes of 19 patients suffering from dry age-related macular degeneration (AMD) (R2 = 0.855). In pseudophakic eyes, maxODx was significantly lower for dry AMD (n = 45) (ODx = 0.491±0.102 ODU) than in eyes with healthy fundus (n = 22) (ODx = 0.615±0.103 ODU) (p = 0.000033). Also in eyes with crystalline lens, maxODx was lower in AMD (n = 125) (ODx = 0.610±0.093 ODU) than in healthy subjects (n = 45) (ODx = 0.674±0.098 ODU) (p = 0.00019). No dependence on age was found in the pseudophakic eyes both of healthy subjects and AMD patients.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Dietrich Schweitzer ; Susanne Jentsch ; Jens Dawczynski ; Martin Hammer ; Ute E.K. Wolf-Schnurrbusch, et al.
"Simple and objective method for routine detection of the macular pigment xanthophyll", J. Biomed. Opt. 15(6), 061714 (December 17, 2010). ; http://dx.doi.org/10.1117/1.3526358


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