Research Papers: Imaging

Adaptive optics multiphoton microscopy to study ex vivo ocular tissues

[+] Author Affiliations
Juan M. Bueno, Emilio J. Gualda, Pablo Artal

Universidad de Murcia, Laboratorio de Óptica, Campus de Espinardo (CiOyN) Murcia, 30100 Spain

J. Biomed. Opt. 15(6), 066004 (December 20, 2010). doi:10.1117/1.3505018
History: Received June 08, 2010; Revised August 27, 2010; Accepted September 10, 2010; Published December 20, 2010; Online December 20, 2010
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We develop an adaptive optics (AO) multiphoton microscope by incorporating a deformable mirror and a Hartmann-Shack wavefront sensor. The AO module operating in closed-loop is used to correct for the aberrations of the illumination laser beam. This increases the efficiency of the nonlinear processes in reducing tissue photodamage, improves contrast, and enhances lateral resolution in images of nonstained ocular tissues. In particular, the use of AO in the multiphoton microscope provides a better visualization of ocular structures, which are relevant in ophthalmology. This instrument might be useful to explore the possible connections between changes in ocular structures and the associated pathologies.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Juan M. Bueno ; Emilio J. Gualda and Pablo Artal
"Adaptive optics multiphoton microscopy to study ex vivo ocular tissues", J. Biomed. Opt. 15(6), 066004 (December 20, 2010). ; http://dx.doi.org/10.1117/1.3505018


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