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Research Papers: Imaging

Spectral Doppler optical coherence tomography imaging of localized ischemic stroke in a mouse model

[+] Author Affiliations
Lingfeng Yu, Elaine Nguyen, Gangjun Liu, Bernard Choi, Zhongping Chen

University of California Irvine, Beckman Laser Institute, Department of Biomedical Engineering, Irvine California 92612-1475

J. Biomed. Opt. 15(6), 066006 (December 07, 2010). doi:10.1117/1.3505016
History: Received April 22, 2010; Revised August 22, 2010; Accepted September 10, 2010; Published December 07, 2010; Online December 07, 2010
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We report the use of spectral Doppler optical coherence tomography imaging (SDOCTI) for quantitative evaluation of dynamic blood circulation before and after a localized ischemic stroke in a mouse model. Rose Bengal photodynamic therapy (PDT) is used as a noninvasive means for inducing localized ischemia in cortical microvasculature of the mouse. Fast, repeated Doppler optical coherence tomography scans across vessels of interest are performed to record flow dynamic information with high temporal resolution. Doppler-angle-independent flow indices are used to quantify vascular conditions before and after the induced ischemia by the photocoagulation of PDT. The higher (or lower) flow resistive indices are associated with higher (or lower) resistance states that are confirmed by laser speckle flow index maps (of laser speckle imaging). Our in vivo experiments shows that SDOCTI can provide complementary quantified flow information that is an alternative to blood volume measurement, and can be used as a means for cortical microvasculature imaging well suited for small animal studies.

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© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

Lingfeng Yu ; Elaine Nguyen ; Gangjun Liu ; Bernard Choi and Zhongping Chen
"Spectral Doppler optical coherence tomography imaging of localized ischemic stroke in a mouse model", J. Biomed. Opt. 15(6), 066006 (December 07, 2010). ; http://dx.doi.org/10.1117/1.3505016


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