Special Section on Pioneers in Biomedical Optics: Michael Feld

Effect of photobleaching on calibration model development in biological Raman spectroscopy

[+] Author Affiliations
Ishan Barman, Chae-Ryon Kong, Ramachandra R. Dasari

Massachusetts Institute of Technology, G. R. Harrison Spectroscopy Laboratory, Laser Biomedical Research Center, Cambridge, Massachusetts 02139

Gajendra P. Singh

University of St. Andrews, School of Physics and Astronomy, St. Andrew KY16 9SS, Scotland

J. Biomed. Opt. 16(1), 011004 (January 07, 2011). doi:10.1117/1.3520131
History: Received March 17, 2010; Revised May 14, 2010; Accepted June 23, 2010; Published January 07, 2011; Online January 07, 2011
Text Size: A A A

A major challenge in performing quantitative biological studies using Raman spectroscopy lies in overcoming the influence of the dominant sample fluorescence background. Moreover, the prediction accuracy of a calibration model can be severely compromised by the quenching of the endogenous fluorophores due to the introduction of spurious correlations between analyte concentrations and fluorescence levels. Apparently, functional models can be obtained from such correlated samples, which cannot be used successfully for prospective prediction. This work investigates the deleterious effects of photobleaching on prediction accuracy of implicit calibration algorithms, particularly for transcutaneous glucose detection using Raman spectroscopy. Using numerical simulations and experiments on physical tissue models, we show that the prospective prediction error can be substantially larger when the calibration model is developed on a photobleaching correlated dataset compared to an uncorrelated one. Furthermore, we demonstrate that the application of shifted subtracted Raman spectroscopy (SSRS) reduces the prediction errors obtained with photobleaching correlated calibration datasets compared to those obtained with uncorrelated ones.

Figures in this Article
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Ishan Barman ; Chae-Ryon Kong ; Gajendra P. Singh and Ramachandra R. Dasari
"Effect of photobleaching on calibration model development in biological Raman spectroscopy", J. Biomed. Opt. 16(1), 011004 (January 07, 2011). ; http://dx.doi.org/10.1117/1.3520131


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.