Special Section on Pioneers in Biomedical Optics: Michael Feld

Video-rate tomographic phase microscopy

[+] Author Affiliations
Christopher Fang-Yen, Yongjin Sung, Charles J. Holbrow, Ramachandra R. Dasari, Michael S. Feld

Massachusetts Institute of Technology, G. R. Harrison Spectroscopy Laboratory, Cambridge, Massachusetts 02139

Wonshik Choi

Korea University, Department of Physics, Seoul 136-701, Republic of Korea

J. Biomed. Opt. 16(1), 011005 (January 14, 2011). doi:10.1117/1.3522506
History: Received May 04, 2010; Revised June 08, 2010; Accepted June 23, 2010; Published January 14, 2011; Online January 14, 2011
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Tomographic phase microscopy measures the 3-D refractive index distribution of cells and tissues by combining the information from a series of angle-dependent interferometric phase images. In the original device, the frame rate was limited to 0.1 frames per second (fps) by the technique used to acquire phase images, preventing measurements of moving or rapidly changing samples. We describe an improved tomographic phase microscope in which phase images are acquired via a spatial fringe pattern demodulation method, enabling a full tomogram acquisition rate of 30 fps. In addition, in this system the refractive index is calculated by a diffraction tomography algorithm that accounts for the effects of diffraction in the 3-D reconstruction. We use the instrument to quantitatively monitor rapid changes in refractive index within defined subregions of cells due to exposure to acetic acid or changes in medium osmolarity.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Christopher Fang-Yen ; Wonshik Choi ; Yongjin Sung ; Charles J. Holbrow ; Ramachandra R. Dasari, et al.
"Video-rate tomographic phase microscopy", J. Biomed. Opt. 16(1), 011005 (January 14, 2011). ; http://dx.doi.org/10.1117/1.3522506


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