Special Section on Pioneers in Biomedical Optics: Michael Feld

Integrated system for combined Raman spectroscopy–spectral domain optical coherence tomography

[+] Author Affiliations
Chetan A. Patil, Anita Mahadevan-Jansen

Vanderbilt University, Department of Biomedical Engineering, Station B, Box 351631, Nashville, Tennessee 37235

Jeroen Kalkman

University of Amsterdam, Academic Medical Center, Biomedical Engineering and Physics Department, PO Box 22700, 1100 DE, Amsterdam, The Netherlands

Dirk J. Faber

University of Amsterdam, Academic Medical Center, Biomedical Engineering and Physics Department, PO Box 22700, 1100 DE, Amsterdam, The Netherlands

University of Amsterdam, Academic Medical Center, Ophthalmology Department, PO Box 22700, 1100 DE, Amsterdam, The Netherlands

Jeffry S. Nyman

Tennessee Valley Healthcare System, Department of Veterans Affairs, Nashville, Tennessee 37212

Vanderbilt University Medical Center, Vanderbilt Center for Bone Biology, Nashville, Tennessee 37232

Vanderbilt University Medical Center, Department of Orthopaedics and Rehabilitation, Nashville, Tennessee 37232

Ton G. van Leeuwen

University of Amsterdam, Academic Medical Center, Biomedical Engineering and Physics Department, PO Box 22700, 1100 DE, Amsterdam, The Netherlands

University of Twente, MIRA Institute for Biomedical Technology and Technical Medicine, Biomedical Photonic Imaging Group, PO Box 217, NL-7500 AE Enschede, The Netherlands

J. Biomed. Opt. 16(1), 011007 (January 07, 2011). doi:10.1117/1.3520132
History: Received May 13, 2010; Revised August 06, 2010; Accepted August 09, 2010; Published January 07, 2011; Online January 07, 2011
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Raman spectroscopy (RS) and optical coherence tomography (OCT) are powerful tools for optical analysis of tissues with mutually complementary strengths and limitations. OCT excels at visualizing tissue microstructure but lacks molecular specificity, while RS can relay tissue biochemical composition but typically cannot relate microstructure. Previous implementations of combined RS-OCT have utilized a common sample arm while maintaining independent RS and OCT detection arms. We present the design and application of an integrated RS-OCT instrument with a common detection arm for both RS and OCT. The detector is a spectrograph capable of sequential detection of the 855-nm OCT signal and the Raman scatter generated by a 785-nm source. The capabilities of the instrument are demonstrated ex vivo in the calvaria and retina of rodents, as well as in vivo in human skin.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Chetan A. Patil ; Jeroen Kalkman ; Dirk J. Faber ; Jeffry S. Nyman ; Ton G. van Leeuwen, et al.
"Integrated system for combined Raman spectroscopy–spectral domain optical coherence tomography", J. Biomed. Opt. 16(1), 011007 (January 07, 2011). ; http://dx.doi.org/10.1117/1.3520132


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