Research Papers: Imaging

Two-dimensional singlet oxygen imaging with its near-infrared luminescence during photosensitization

[+] Author Affiliations
Bolin Hu, Nan Zeng, Zhiyi Liu, Weidong Xie, Yong Zhou, Yonghong He, Hui Ma

Tsinghua University, Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Shenzhen, 518055, China

Yanhong Ji

China Normal University, MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, South Guangzhou 510631, China

Qing Peng

Shanghai Jiaotong University, Xinhua Hospital, School of Medicine, Department of Ophthalmology, Shanghai 200092, China

J. Biomed. Opt. 16(1), 016003 (January 12, 2011). doi:10.1117/1.3528593
History: Received June 25, 2010; Revised November 07, 2010; Accepted November 09, 2010; Published January 12, 2011; Online January 12, 2011
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Photodynamic therapy is a promising cancer treatment that involves activation of photosensitizer by visible light to create singlet oxygen. This highly reactive oxygen species is believed to induce cell death and tissue destruction in PDT. Our approach used a near-infrared area CCD with high quantum efficiency to detect singlet oxygen by its 1270-nm luminescence. Two-dimensional singlet oxygen images with its near-infrared luminescence during photosensitization could be obtained with a CCD integration time of 1 s, without scanning. Thus this system can produce singlet oxygen luminescence images faster and achieve more accurate measurements in comparison to raster-scanning methods. The experimental data show a linear relationship between the singlet oxygen luminescence intensity and sample concentration. This method provides a detection sensitivity of 0.0181 μg/ml (benzoporphyrin derivative monoacid ring A dissolved in ethanol) and a spatial resolution better than 50 μm. A pilot study was conducted on a total of six female Kunming mice. The results from this study demonstrate the system's potential for in vivo measurements. Further experiments were carried out on two tumor-bearing nude mice. Singlet oxygen luminescence images were acquired from the tumor-bearing nude mouse with intravenous injection of BPD-MA, and the experimental results showed real-time singlet oxygen signal depletion as a function of the light exposure.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Bolin Hu ; Nan Zeng ; Zhiyi Liu ; Yanhong Ji ; Weidong Xie, et al.
"Two-dimensional singlet oxygen imaging with its near-infrared luminescence during photosensitization", J. Biomed. Opt. 16(1), 016003 (January 12, 2011). ; http://dx.doi.org/10.1117/1.3528593


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