Special Section on Coherent Raman Imaging Techniques and Biomedical Applications

Video-rate wide-field coherent anti-Stokes Raman scattering microscopy with collinear nonphase-matching illumination

[+] Author Affiliations
Ming Lei

University of Konstanz, Department of Chemistry, D-78457 Konstanz, Germany

Chinese Academy of Sciences, Xi'an Institute of Optics and Precision Mechanics, State Key Laboratory of Transient Optics and Photonics, Xi'an 710119, China

Martin Winterhalder, Romedi Selm, Andreas Zumbusch

University of Konstanz, Department of Chemistry, D-78457 Konstanz, Germany

J. Biomed. Opt. 16(2), 021102 (February 10, 2011). doi:10.1117/1.3533707
History: Received June 03, 2010; Revised June 25, 2010; Accepted June 28, 2010; Published February 10, 2011; Online February 10, 2011
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A simple scheme for video-rate wide-field coherent anti-Stokes Raman scattering (CARS) microscopy is presented. The method is based on collinear nonphase-matching illumination. The mechanisms leading to CARS signal generation are investigated. We find that refraction-mediated phase-matching is the main effect. Video-rate wide-field CARS microscopy of polystyrene beads and CARS wide-field images of C. elegans embryos are shown, and the capabilities and the limitations of the scheme are discussed.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Ming Lei ; Martin Winterhalder ; Romedi Selm and Andreas Zumbusch
"Video-rate wide-field coherent anti-Stokes Raman scattering microscopy with collinear nonphase-matching illumination", J. Biomed. Opt. 16(2), 021102 (February 10, 2011). ; http://dx.doi.org/10.1117/1.3533707


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