Special Section on Coherent Raman Imaging Techniques and Biomedical Applications

Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy

[+] Author Affiliations
Aaron D. Slepkov, Andrew Ridsdale, Douglas J. Moffatt, John P. Pezacki

National Research Council of Canada, Steacie Institute for Molecular Sciences, Ottawa, Ontario, K1A 0R6 Canada

Huei-Ning Wan, Ming-Hao Wang, Fu-Jen Kao

National Yang-Ming University, Institute of Biophotonics, 155, Li-Nong Street, Section 2, Taipei 11221, Taiwan

Adrian F. Pegoraro, Albert Stolow

National Research Council of Canada, Steacie Institute for Molecular Sciences, Ottawa, Ontario, K1A 0R6 Canada

Queen's University, Department of Physics, 99 University Avenue, Kingston, Ontario K7L 3N6 Canada

J. Biomed. Opt. 16(2), 021103 (February 01, 2011). doi:10.1117/1.3490641
History: Received March 29, 2010; Revised May 28, 2010; Accepted June 02, 2010; Published February 01, 2011; Online February 01, 2011
Text Size: A A A

We demonstrate the simultaneous collection and separation of femtosecond-laser-based forward-collected coherent anti-Stokes Raman scattering (F-CARS) and two-photon-excitation-induced fluorescence lifetime images (FLIM) using time-correlated single photon counting (TCSPC). We achieve this in a nondescanned geometry using a single multimode fiber without significant loss of light, field of view, and most importantly, TCSPC timing fidelity. In addition to showing the ability to separate CARS images from FLIM images using time gating, we also demonstrate composite multimodal epicollected FLIM imaging with fiber-collected F-CARS imaging in live cells.

Figures in this Article
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Aaron D. Slepkov ; Andrew Ridsdale ; Huei-Ning Wan ; Ming-Hao Wang ; Adrian F. Pegoraro, et al.
"Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy", J. Biomed. Opt. 16(2), 021103 (February 01, 2011). ; http://dx.doi.org/10.1117/1.3490641


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.